A phospholipase C inhibitor, phenylmethylsulfonyl fluoride, ameliorates ischemic injury to brain mitochondria in rats.

To study the effect of phenylmethylsulfonyl fluoride (PMSF), a phospholipase C inhibitor, on ischemic injury to brain mitochondria in rats. The phospholipid level, membrane fluidity, and respiratory control ratio of brain mitochondria were measured. The effect of phenylmethylsulfonyl fluoride was tested. The phospholipid level, membrane fluidity, and respiratory control ratio of brain mitochondria in ischemia group decreased but increased in the PMSF treatment group (P < 0.05). PMSF ameliorated ischemic injury to brain mitochondria in rats.