跳转至内容
Merck
  • RBM28, a protein deficient in ANE syndrome, regulates hair follicle growth via miR-203 and p63.

RBM28, a protein deficient in ANE syndrome, regulates hair follicle growth via miR-203 and p63.

Experimental dermatology (2015-05-06)
Emily Warshauer, Liat Samuelov, Ofer Sarig, Dan Vodo, Albrecht Bindereif, Moien Kanaan, Uri Gat, Dana Fuchs-Telem, Noam Shomron, Luba Farberov, Metsada Pasmanik-Chor, Gil Nardini, Eyal Winkler, Benjamin Meilik, Isabelle Petit, Daniel Aberdam, Ralf Paus, Eli Sprecher, Janna Nousbeck
摘要

Alopecia-neurological defects-endocrinopathy (ANE) syndrome is a rare inherited hair disorder, which was shown to result from decreased expression of the RNA-binding motif protein 28 (RBM28). In this study, we attempted to delineate the role of RBM28 in hair biology. First, we sought to obtain evidence for the direct involvement of RBM28 in hair growth. When RBM28 was downregulated in human hair follicle (HF) organ cultures, we observed catagen induction and HF growth arrest, indicating that RBM28 is necessary for normal hair growth. We also aimed at identifying molecular targets of RBM28. Given that an RBM28 homologue was recently found to regulate miRNA biogenesis in C. elegans and given the known pivotal importance of miRNAs for proper hair follicle development, we studied global miRNA expression profile in cells knocked down for RBM28. This analysis revealed that RBM28 controls the expression of miR-203. miR-203 was found to regulate in turn TP63, encoding the transcription factor p63, which is critical for hair morphogenesis. In conclusion, RBM28 contributes to HF growth regulation through modulation of miR-203 and p63 activity.

材料
货号
品牌
产品描述

Sigma-Aldrich
荧光素, for fluorescence, free acid
Sigma-Aldrich
硫代硫酸钠, ≥99.99% trace metals basis
Sigma-Aldrich
3-氨基-9-乙基咔唑, ≥95% (HPLC), powder
Sigma-Aldrich
3-氨基-9-乙基咔唑, tablet
Sigma-Aldrich
硫代硫酸钠 溶液, 0.1 M
Sigma-Aldrich
三(叔丁氧基)硅烷醇, 99.999%
Sigma-Aldrich
硫代硫酸钠 溶液, 0.01 M
Sigma-Aldrich
硫代硫酸钠 溶液, 2 g/dL in deionized water
Sigma-Aldrich
硫代硫酸钠 溶液, 0.025 M
Sigma-Aldrich
硫代硫酸钠 溶液, 1 M
Sigma-Aldrich
MISSION® esiRNA, targeting mouse Rbm28
Sigma-Aldrich
MISSION® esiRNA, targeting human RBM28