跳转至内容
Merck
  • MicroRNA-155 negatively affects blood-brain barrier function during neuroinflammation.

MicroRNA-155 negatively affects blood-brain barrier function during neuroinflammation.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology (2014-03-08)
Miguel Alejandro Lopez-Ramirez, Dongsheng Wu, Gareth Pryce, Julie E Simpson, Arie Reijerkerk, Josh King-Robson, Oliver Kay, Helga E de Vries, Mark C Hirst, Basil Sharrack, David Baker, David Kingsley Male, Gregory J Michael, Ignacio Andres Romero
摘要

Blood-brain barrier (BBB) dysfunction is a hallmark of neurological conditions such as multiple sclerosis (MS) and stroke. However, the molecular mechanisms underlying neurovascular dysfunction during BBB breakdown remain elusive. MicroRNAs (miRNAs) have recently emerged as key regulators of pathogenic responses, although their role in central nervous system (CNS) microvascular disorders is largely unknown. We have identified miR-155 as a critical miRNA in neuroinflammation at the BBB. miR-155 is expressed at the neurovascular unit of individuals with MS and of mice with experimental autoimmune encephalomyelitis (EAE). In mice, loss of miR-155 reduced CNS extravasation of systemic tracers, both in EAE and in an acute systemic inflammation model induced by lipopolysaccharide. In cultured human brain endothelium, miR-155 was strongly and rapidly upregulated by inflammatory cytokines. miR-155 up-regulation mimicked cytokine-induced alterations in junctional organization and permeability, whereas inhibition of endogenous miR-155 partially prevented a cytokine-induced increase in permeability. Furthermore, miR-155 modulated brain endothelial barrier function by targeting not only cell-cell complex molecules such as annexin-2 and claudin-1, but also focal adhesion components such as DOCK-1 and syntenin-1. We propose that brain endothelial miR-155 is a negative regulator of BBB function that may constitute a novel therapeutic target for CNS neuroinflammatory disorders.

材料
货号
品牌
产品描述

Sigma-Aldrich
丙酮, ACS reagent, ≥99.5%
货号
包装规格
是否有货
价格
数量
Sigma-Aldrich
丙酮, suitable for HPLC, ≥99.9%
货号
包装规格
是否有货
价格
数量
Sigma-Aldrich
丙酮, HPLC Plus, for HPLC, GC, and residue analysis, ≥99.9%
货号
包装规格
是否有货
价格
数量
Sigma-Aldrich
丙酮, Laboratory Reagent, ≥99.5%
货号
包装规格
是否有货
价格
数量
Sigma-Aldrich
丙酮, puriss. p.a., ACS reagent, reag. ISO, reag. Ph. Eur., ≥99.5% (GC)
货号
包装规格
是否有货
价格
数量
Sigma-Aldrich
丙酮, suitable for HPLC, ≥99.8%
货号
包装规格
是否有货
价格
数量
Sigma-Aldrich
丙酮, ACS reagent, ≥99.5%
货号
包装规格
是否有货
价格
数量
USP
丙酮, United States Pharmacopeia (USP) Reference Standard
货号
包装规格
是否有货
价格
数量
Sigma-Aldrich
丙酮, JIS special grade, ≥99.5%
货号
包装规格
是否有货
价格
数量
Sigma-Aldrich
丙酮, histological grade, ≥99.5%
货号
包装规格
是否有货
价格
数量
Sigma-Aldrich
丙酮, puriss., meets analytical specification of Ph. Eur., BP, NF, ≥99% (GC)
货号
包装规格
是否有货
价格
数量
Sigma-Aldrich
丙酮, natural, ≥97%
货号
包装规格
是否有货
价格
数量
Sigma-Aldrich
丙酮, SAJ first grade, ≥99.0%
货号
包装规格
是否有货
价格
数量
Sigma-Aldrich
丙酮, ≥99%, meets FCC analytical specifications
货号
包装规格
是否有货
价格
数量
Sigma-Aldrich
丙酮, ≥99.5%, for residue analysis
货号
包装规格
是否有货
价格
数量
Supelco
丙酮, analytical standard
货号
包装规格
是否有货
价格
数量
Sigma-Aldrich
丙酮, for residue analysis, ≥99.5%
货号
包装规格
是否有货
价格
数量
Supelco
丙酮, Pharmaceutical Secondary Standard; Certified Reference Material
货号
包装规格
是否有货
价格
数量
Sigma-Aldrich
丙酮, for chromatography, ≥99.8%
货号
包装规格
是否有货
价格
数量
Sigma-Aldrich
丙酮, for residue analysis, JIS 5000
货号
包装规格
是否有货
价格
数量
Sigma-Aldrich
丙酮, suitable for HPLC, ≥99.9%
货号
包装规格
是否有货
价格
数量
Sigma-Aldrich
丙酮, suitable for HPLC
货号
包装规格
是否有货
价格
数量