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Merck
  • Overexpression, purification, crystallization and preliminary X-ray crystal analysis of Bacillus pallidusD-arabinose isomerase.

Overexpression, purification, crystallization and preliminary X-ray crystal analysis of Bacillus pallidusD-arabinose isomerase.

Acta crystallographica. Section F, Structural biology and crystallization communications (2008-10-22)
Kosei Takeda, Hiromi Yoshida, Goro Takada, Ken Izumori, Shigehiro Kamitori
摘要

D-Arabinose isomerase catalyzes the isomerization of D-arabinose to D-ribulose. Bacillus pallidus D-arabinose isomerase has broad substrate specificity and can catalyze the isomerization of D-arabinose, L-fucose, L-xylose, L-galactose and D-altrose. Recombinant B. pallidus D-arabinose isomerase was overexpressed, purified and crystallized. A crystal of the enzyme was obtained by the sitting-drop method at room temperature and belonged to the orthorhombic space group P2(1)2(1)2, with unit-cell parameters a = 144.9, b = 127.9, c = 109.5 A. Diffraction data were collected to 2.3 A resolution.

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Sigma-Aldrich
L-Ribulose, ≥90% (HPLC)
Sigma-Aldrich
D-核酮糖 溶液, ~1 M in H2O, ≥97.0% (HPLC)