High-frequency transfection of CHO cells using polybrene.

High-frequency transfection of CHO cells using polybrene.

Somatic cell and molecular genetics (1986-05-01)

W G Chaney, D R Howard, J W Pollard, S Sallustio, P Stanley

摘要

High-frequency transfection of CHO cells has been achieved for several plasmids, a cosmid library, and genomic DNA using Polybrene and dimethyl sulfoxide. All plasmid transfectants examined were stable and exhibited plasmid sequences in genomic DNA. The method is simple, reproducible, and succeeded with several independent CHO clones in the presence or the absence of carrier DNA, even at very low concentrations of plasmid DNA.

材料

货号

品牌

产品描述

TR-1003

Sigma-Aldrich

聚乙烯感染/转染试剂, A highly efficient method of gene transfer into mammalian cells leveraging infection with retroviral vectors.