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Induction of direct somatic embryogenesis in garlic (Allium sativum).

Methods in cell science : an official journal of the Society for In Vitro Biology (2001-09-11)
S J Sata, S B Bagatharia, V S Thaker
摘要

An efficient novel method of direct somatic embryogenesis from basal tissue of garlic clove was developed. The influence of plant growth regulators, basal medium and explant type on somatic embryo induction was examined. The best plant growth regulator combination was, 2,4-D and kinetin at 1.0 mg/L and 0.5 mg/L respectively, inducing direct somatic embryogenesis in 60% of explants. White's medium was used as basal medium and somatic embryos developed on explants after six weeks. The technique has potential applicability for problems associated with plant regeneration and virus elimination in garlic.

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White 基本混合盐, powder, suitable for plant cell culture
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