跳转至内容
Merck
HomeProductsMolecular Biology & Functional GenomicsMolecular BiologyRNA Purification & Isolation Kits

RNA Purification & Isolation Kits

Purification kits for RNA extraction and isolation

RNA purification is a critical first step in many analyses due to the easy degradability of RNA. GenElute™ RNA purification kits enable purification of RNA from a variety of sources quickly and efficiently with minimal degradation, maximum purity, and high recovery and yield. GenElute™-E Single Spin RNA purification kits can be used to clean up RNA preps in a single spin for PCR and downstream applications.

Related Product Literature
Nucleic Acid Purification Product Guide
Viral RNA Purification Protocol Using GenElute™ Spin Prep Kits
Instructions for purifying viral RNA using GenElute™ RNA purification spin prep kits.

GenElute™ RNA Purification Kits

Classic GenElute™ RNA purification kits use silica or silicon carbide to strongly bind RNA, allowing proteins and other biological contaminants to be removed through wash steps. mRNA purification kits use oligo dT30 covalently linked to polystyrene beads to capture polyadenylated mRNA by hybridization. After washing, purified RNA is eluted. A broad portfolio of GenElute™ purification kits tailored to specific sample types are available, enabling purification of total RNA and mRNA from cells, tissues, serum, plasma, bacteria, viruses, and plant tissue.

Features:

  • High yield, high purity RNA with minimal degradation
  • Comprehensive portfolio of kits optimized for purification of total RNA or mRNA from a variety of sample types
  • Offered in a variety of sizes and formats, from miniprep, midiprep, and maxiprep kits to high-throughput 96-well kits
  • Includes kits for RNA/DNA co-purification
  • Vacuum and spin formats

GenElute™-E single Spin RNA Purification Kits

GenElute™-E RNA purification kits use a negative chromatography technique dependent on size exclusion to isolate and purify nucleic acid in a single spin. Smaller molecules such as proteins become trapped in the pores of the stationary phase of the column. Larger RNA molecules flow through the column quickly because they are too large to enter the pores. By exploiting this difference in size, GenElute™-E purification kits enable RNA purification in a single spin without the need for multiple binding and wash steps.

Features:

  • Negative chromatography RNA purification method based on size exclusion
  • Simplified workflow
  • Quick protocol (3 minutes hands-on time, for a total of 15 minutes prep time)
  • Fewer chemical impurities, with no chaotropic salts or alcohol wash solutions that can inhibit downstream processes such as PCR
  • Reduced plastic waste and no hazardous binding or wash buffers
  • Kits tailored for RNA cleanup

RNA Purification Essentials

A variety of reagents are available to protect RNA from degradation. Stabilyser™ reagent is a proprietary formulation of detergents and solutes optimized for efficient extraction, stabilization, and storage of protein, DNA, and RNA from tissue samples. RNaseZAP™ reagent is a cleaning agent for removing RNase from glassware, plastic surfaces, countertops, and pipettors. RNAlater® reagent is an aqueous, non-toxic tissue storage reagent that rapidly permeates tissue to stabilize and protect cellular RNA through RNase inactivation. RNAstable® reagent is used to coat the bottom of storage tubes or wells, protecting picogram to microgram amounts of RNA at room temperature.

Related Product Categories
Nucleic Acid Electrophoresis, Transfer, and Quantitation
DNA and RNA gel electrophoresis reagents and Northern and Southern blotting tools for molecular biology and genomics research.
PCR Reagents & Kits
PCR Reagents & Kits
Custom & Predesigned DNA Oligos and qPCR Probes
Discover our custom DNA oligos portfolio including primers, qPCR probes, high-throughput DNA in plates, and next-generation sequencing (NGS) adapters available in multiple formats that comply with ISO 9001 and ISO 13485 quality systems for research and commercial applications.
Plasmid Purification & Isolation Kits
Plasmid DNA purification kits offered in miniprep, midiprep, maxiprep, and gigaprep sizes for small to large scale purification of plasmid DNA for molecular cloning, transformation, and recombinant protein expression applications.
gDNA Purification & Isolation Kits
High yield, high quality DNA extraction and purification kits tailored to a variety of common and challenging sample types including cell culture, mammalian tissue, blood, viruses, bacteria, plant tissue, soil, water, urine, stool, PCR reactions, and agarose gel extractions.
Related Applications
DNA & RNA Purification
Overview of common techniques and downstream applications for extraction and purification of genomic DNA, plasmid DNA, and total RNA from cells, tissue, blood, viruses, and other sample types.
Nucleic Acid Gel Electrophoresis
Learn the basics of DNA and RNA gel electrophoresis, and how these techniques can be used to separate and purify DNA and RNA molecules based on size and charge for cloning, PCR, mass spectrometry, next generation sequencing (NGS), and Northern and Southern blotting applications.
PCR Applications
Polymerase chain reaction (PCR) is a technique for amplifying nucleic acid molecules and is commonly used in many applications, including RT-PCR, hot start PCR, end point PCR and more.
qPCR
Quantitative PCR (qPCR) or quantitative Real-Time PCR applications, utilizing fluorescent report molecules, suitable for your microRNA, genotyping, and gene expression analyses.
Nucleic Acid Labeling & Detection
DNA and RNA nucleic acid labeling and detection methods and applications for northern blot, Southern blot, and additional nucleic acid detection assays.
Functional Genomics Screening
Functional genomics screening principles using RNAi and CRISPR/Cas9 libraries for drug target identification.
登录以继续。

如要继续阅读，请登录或创建帐户。

暂无帐户？