N2635

Nucleoside 5′-Diphosphate Kinase from bovine liver

buffered aqueous glycerol solution, ≥1,000 units/mg protein (biuret)

• 别名: NDK, UDPkinase, nonmetastatic23(NM23), uridine diphosphate kinase, ATP:nucleoside diphosphate phosphotransferase, NDPK
• CAS号: 9026-51-1
• EC 号: 2.7.4.6 (BRENDA, IUBMB)
• MDL號碼: MFCD00131734
• 分類程式碼代碼: 12352204
• eCl@ss: 32160410
• NACRES: NA.54

属性

• 生物源: bovine liver
• 品質等級: 200
• 形狀: buffered aqueous glycerol solution
• 比活性: ≥1,000 units/mg protein (biuret)
• 儲存條件: (Tightly closed)
• 技術: activity assay: suitable
• 異物活動: lactic dehydrogenase, myokinase, β-NADH oxidase, nucleoside monophosphokinase and ATPase ≤0.1%
• 儲存溫度: 2-8°C

说明

一般說明

Research area: Cell Signaling

Nucleoside 5′-Diphosphate Kinase (NDK) is a ubiquitous housekeeping enzyme. Nucleoside Diphosphate Kinase exists in two isoforms in eukaryotic cells, NDK-A and NDK-B. These enzymes are found expressed both in the mitochondria and the cytoplasm.

應用

Nucleoside 5′-Diphosphate Kinase has been used:

• nucleoside diphosphate kinase (NDPK) assays
• coupled NDPK-luciferase assay to determine the amounts of Ras-bound guanosine triphosphate (GTP)
• non-metastatic protein 23 (NM23) growth stimulation assay
• in a study to assess inhibition of type I Fc epsilon receptor mediated Ca²⁺ influx and mediator secretion in rat mucosal mast cells
• in a study to investigate protein synthesis in rabbit reticulocytes

生化／生理作用

Nucleoside 5′-Diphosphate Kinases (NDKs) aid in the synthesis of nucleoside triphosphates (NTPs) by transferring a phosphate group from ATP to nucleoside diphosphates (NDPs). NDKs provide NTPs for nucleic acid synthesis, cytidine 5′-triphosphate (CTP) for lipid synthesis, uridine 5′-triphosphate (UTP) for polysaccharide synthesis, and guanosine triphosphate (GTP) for protein elongation, signal transduction, and microtubule polymerization.

單位定義

One unit will convert 1.0 μmole each of TDP and ATP to TTP and ADP per min at pH 7.6 at 25 °C in a coupled system with PK/LDH.

安全信息

• 儲存類別代碼: 10 - Combustible liquids
• 水污染物質分類（WGK）: WGK 3
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable