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生物源
mouse
品質等級
共軛
unconjugated
抗體表格
purified antibody
抗體產品種類
primary antibodies, primary antibodies
無性繁殖
125-10, monoclonal
純化經由
using protein G
物種活性
human
包裝
antibody small pack of 100 μg
技術
immunocytochemistry: suitable
immunofluorescence: suitable
同型
IgG1κ
表位序列
Unknown
運輸包裝
dry ice
目標翻譯後修改
unmodified
一般說明
The nucleoli small basophilic, spherical, non-membrane enclosed, highly conserved, structures that are localized within the nucleus. Usually, they are localized in the central nuclear region or close to the nuclear membrane. They are formed around nucleolus organizer regions (NORs) consisting of ribosomal DNA (rDNA). They are structurally organized into the fibrillar center, the dense fibrillar component, and the granular component region. Clone 125-10 detects human nucleoli by immunocytochemistry.
特異性
Clone 125-10 is a mouse monoclonal antibody that specifically detects nucleoli in human cells.
免疫原
Raji cell nuclei.
應用
Quality Control Testing
Evaluated by Immunocytochemistry in A431 cells.
Immunocytochemistry Analysis: A 1:1,000 dilution of this antibody detected Nucleoli in A431 cells.
Tested Applications
Immunocytochemistry Analysis: A representative lot detected Nucleoli in Immunocytochemistry applications (Endo, A., et al. (2009). J Biol Chem. 284(41):27918-27923; Endo, A., et al. (2009). J Cell Sci. 122(Pt 5):678-86).
Immunofluorescence Analysis: A representative lot detected Nucleoli in Immunofluorescence applications (Clements, D., et al. (2007). Am J Physiol Lung Cell Mol Physiol. 292(1):L258-66; Vallee, D., et al. (2004). J Med Genet. 41(10):778-83; Ohno, M., et al. (2002). Chromosoma. 111(3):201-13).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Evaluated by Immunocytochemistry in A431 cells.
Immunocytochemistry Analysis: A 1:1,000 dilution of this antibody detected Nucleoli in A431 cells.
Tested Applications
Immunocytochemistry Analysis: A representative lot detected Nucleoli in Immunocytochemistry applications (Endo, A., et al. (2009). J Biol Chem. 284(41):27918-27923; Endo, A., et al. (2009). J Cell Sci. 122(Pt 5):678-86).
Immunofluorescence Analysis: A representative lot detected Nucleoli in Immunofluorescence applications (Clements, D., et al. (2007). Am J Physiol Lung Cell Mol Physiol. 292(1):L258-66; Vallee, D., et al. (2004). J Med Genet. 41(10):778-83; Ohno, M., et al. (2002). Chromosoma. 111(3):201-13).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-Nucleoli, clone 125-10, Cat. No. MAB1277-I, is a mouse monoclonal antibody that detects nucleoli and is tested for use in Immunocytochemistry, Immunofluorescence, and Immunocytochemistry.
外觀
Purified mouse monoclonal antibody IgG1 in PBS without preservatives.
儲存和穩定性
Store at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
其他說明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
儲存類別代碼
12 - Non Combustible Liquids
水污染物質分類（WGK）
WGK 2
閃點（°F）
Not applicable
閃點（°C）
Not applicable
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