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Key Documents

03-0184-00

Millipore

SMC® Human Phospho-Tau (T181) High Sensitivity Immunoassay Kit

别名:

pTau T181 Single Molecule Counting Kit, pTau T181 immunoassay kit, pTau T181 ultra-sensitive immunoassay, pTau Thr181 immunoassay kit

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About This Item

分類程式碼代碼:
12161503

製造商/商標名

SMC®

assay range

sensitivity: 0.94 pg/mL
(Lower Limit of Quantification: )

inter-assay cv: ≤12%
intra-assay cv: ≤7%
(five normal plasma samples run in triplicate by 3 different operators on 3 different days.)

技術

single molecule counting: suitable

儲存溫度

2-8°C

一般說明

The SMC® Human Phospho-Tau (T181) High Sensitivity Immunoassay Kit contains all reagents required to perform the assay. This assay takes advantage of a standard immunoassay workflow configured in a standard plate format.
Assay protocols are similar to existing sandwich ELISA methods with two key differences:
1) Elution buffer disrupts the sandwich, separating the labeled detection antibody for quantification.
2) The Erenna and SMCxPRO® immunoassay systems detect analytes using Single Molecule Counting (SMC®) technology.

特異性

Species Reactivity:
Specificity was not tested.

Cross Reactivity:
Cross-reactivity was not tested.

Accuracy:
  • Minimum of 5 serum samples spiked with average recovery of 97% with a range of 70-138%
  • Minimum of 5 plasma samples spiked with average recovery of 97% with a range of 81-127%
  • Minimum of 5 CSF samples spiked with average recovery of 108% with a range of 82-146%

應用

The sensitivity of the Single Molecule Counting (SMC®) technology gives the SMC® Human Phospho-Tau (T181) High Sensitivity Immunoassay Kit unparalleled performance in the quantification of Phospho-Tau (T181) in human serum, plasma, and CSF.

This new streamline kit has the following features including:
  • Improved options for sample prep and incubation
  • Lyophilization of the standard provides the kit at one storage condition
  • Removal of a plate transfer step
  • Increased reagent volumes to accommodate automation
  • Validated to run on both SMCxPRO® and Erenna


Research Category:
  • Neuroscience
  • Neurological


Research Sub-Category:
  • Alzheimer′s Disease
  • Aging
  • Nervous System
  • Neurodegenerative Diseases
  • Neurofilament & Neuron Metabolism
  • Neuronal & Glial Markers
  • Neuroregenerative Medicine
  • Neuroscience

包裝

96 well assay

成分

  • Assay Buffer
  • pTau T181 Coated Beads
  • Standard Diluent
  • pTau T181 Detection Antibody
  • pTau T181 Standard
  • 10X Wash Buffer
  • Buffer D
  • Elution Buffer B

法律資訊

SMC is a registered trademark of Singulex
SMCxPRO is a registered trademark of Merck KGaA, Darmstadt, Germany

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

象形圖

Skull and crossbonesHealth hazard

訊號詞

Danger

危險分類

Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Irrit. 2 - Repr. 1B - STOT RE 2 Inhalation

標靶器官

Respiratory Tract

儲存類別代碼

6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

水污染物質分類(WGK)

WGK 3


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