低压液相色谱
低压液相色谱(LPLC)系统借助低压驱动流动相通过色谱柱。LPLC曾使用开管型色谱柱，借助重力作用将溶剂“泵”入色谱柱中。这种LPLC称为“开管柱液相色谱”。如今，LPLC通常通过泵来驱动仪器。
LPLC技术具有高度选择性，大多用于研究蛋白质、多肽和单克隆抗体等生物分子。LPLC技术基本具备制备能力和非破坏性，非常适于生物分子的分离和重组标签蛋白的纯化。LPLC还用于活性药物成分纯化、样品农药去除以及小分子纯化。
LPLC仪器
LPLC是由色谱柱、低压泵（通常为蠕动泵）、进样器、检测器和馏分收集器组成的系统。简单的蠕动滚子泵以固定流速从贮存器中抽取流动相并转移至色谱柱。
随后溶剂（流动相）流过含样品的进样器，将样品输送到色谱柱中进行分离。样品组分流过色谱柱并以不同速率到达检测器，实现分离。紫外-可见光或折光率(RI)在线检测器监测色谱柱流出物，使样品能被馏分收集器收集。收集样品按洗脱时间排列。
LPLC吸附剂和硬件
柱基质材料应具有良好的机械和化学稳定性，并应含利于分析物吸附的官能团且呈惰性。色谱柱硬件材料通常由玻璃和惰性塑料组成，具体取决于所用的溶剂。
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