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  • Bioluminescent liquid light guide pad biosensor for indoor air toxicity monitoring.

Bioluminescent liquid light guide pad biosensor for indoor air toxicity monitoring.

Analytical chemistry (2015-03-17)
Evgeni Eltzov, Avital Cohen, Robert S Marks
ABSTRACT

Indoor air pollution became a recent concern found to be oftentimes worse than outdoor air quality. We developed a tool that is cheap and simple and enables continuous monitoring of air toxicity. It is a biosensor with both a nondisposable (monitor) and disposable (calcium alginate pads with immobilized bacteria) elements. Various parameters to enhance its signal have been tested (including the effect of the pad's orientation, it's exposure to either temperature or time with the air toxicant analyte, and various concentrations thereof). Lastly, the sensor has demonstrated its ability to sense the presence of chemicals in a real, indoor environment. This is the first step in the creation of a sensitive and simple operative tool that may be used in different indoor environments.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Formaldehyde-12C solution, 20% in H2O, 99.9 atom % 12C
Supelco
1-Octanol, analytical standard
Sigma-Aldrich
Sodium chloride, BioUltra, for molecular biology, ≥99.5% (AT)
Sigma-Aldrich
N-Ethyldiisopropylamine, BASF quality, ≥98.0%
Supelco
Tetrahydrofuran, Selectophore, ≥99.5%
Supelco
N,N-Dimethylformamide, analytical standard
Supelco
Methanol, analytical standard
Sigma-Aldrich
N-Ethyldiisopropylamine solution, suitable for peptide synthesis, ~2 M in 1-methyl-2-pyrrolidinone
Sigma-Aldrich
Formaldehyde solution, tested according to Ph. Eur.
Supelco
Acetone, analytical standard
Sigma-Aldrich
Sodium chloride, tested according to Ph. Eur.
Supelco
Calcium standard for AAS, analytical standard, 1.000 g/L Ca+2 in hydrochloric acid, traceable to BAM
Sigma-Aldrich
Formaldehyde solution, for molecular biology, BioReagent, ≥36.0% in H2O (T)
Supelco
Chloroform, analytical standard
Supelco
Diethyl ether, analytical standard
Sigma-Aldrich
Chloroform, SAJ first grade, ≥99.0%, contains 0.4-0.8% ethanol
Sigma-Aldrich
Diethylene glycol, SAJ first grade, ≥98.0%
Sigma-Aldrich
Formaldehyde solution, JIS special grade, 36.0-38.0%, contains methanol as stabilizer
Sigma-Aldrich
Chloroform, JIS special grade, ≥99.0%
Sigma-Aldrich
Methanol, JIS special grade, ≥99.8%
Sigma-Aldrich
Acetone, for residue analysis, ≥99.5%
Sigma-Aldrich
Chloroform, JIS 300, ≥99.0%, for residue analysis
Sigma-Aldrich
2-Mercaptoethanol, ≥99.0%
Sigma-Aldrich
Diethyl ether, JIS special grade, ≥99.5%
Sigma-Aldrich
N,N-Dimethylformamide, SAJ first grade, ≥99.0%
Sigma-Aldrich
Tetrahydrofuran, SAJ first grade, ≥99.0%
Sigma-Aldrich
2-Mercaptoethanol, SAJ special grade, ≥99.0%
Sigma-Aldrich
1-Octanol, JIS special grade, ≥98.0%
Sigma-Aldrich
Sodium chloride solution, 0.1 M
Sigma-Aldrich
N,N-Dimethylformamide, JIS special grade, ≥99.5%