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Key Documents

V900477

Sigma-Aldrich

HEPES

Vetec, reagent grade, 99.5%

Synonym(s):

4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid, N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid)

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About This Item

Empirical Formula (Hill Notation):
C8H18N2O4S
CAS Number:
Molecular Weight:
238.30
Beilstein:
883043
EC Number:
MDL number:
UNSPSC Code:
12161700
PubChem Substance ID:

grade

reagent grade

product line

Vetec

Assay

99.5%

form

crystalline powder

pH

5.0-6.5 (25 °C, 238 g/L)

useful pH range

6.8-8.2

pKa (25 °C)

7.5

solubility

water: 500 mg/mL, clear, colorless

SMILES string

OCCN1CCN(CC1)CCS(O)(=O)=O

InChI

1S/C8H18N2O4S/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14/h11H,1-8H2,(H,12,13,14)

InChI key

JKMHFZQWWAIEOD-UHFFFAOYSA-N

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General description

HEPES, a zwitterionic buffer having a pH range of 6.8-8.2, is one among the 20 Good′s buffers. It′s commonly used in cell culture applications as it helps in maintaining the physiological pH.

Legal Information

Vetec is a trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Acid-base buffering in organ preservation solutions as a function of temperature: new parameters for comparing buffer capacity and efficiency
Baicu SC and Taylor MJ
Cryobiology, 45(1), 33-48 (2002)
Evidence-based guidelines for controlling pH in mammalian live-cell culture systems
Michl J, et al.
Communications biology, 2(1), 1-12 (2019)
Brendan M Leung et al.
Journal of tissue engineering and regenerative medicine, 9(11), 1247-1258 (2013-03-19)
Modular cardiac tissues developed both vascular and cardiac structures in vivo, provided that the host response was attenuated by omitting xenoproteins from the modules. Collagen gel modules (with Matrigel(TM)) containing cardiomyocytes (CMs) alone or CMs with surface-seeded endothelial cells (ECs; CM/EC
P Dey et al.
Oncogene, 33(33), 4213-4225 (2013-10-01)
Estrogen receptor β (ERβ) is emerging as a critical factor in understanding prostate cancer biology. Although reduced in prostate cancer above Gleason grade 3, ERβ is a potential drug target at the initial stage of the disease. In human prostate
Frédéric Perros et al.
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Endothelial cell (EC) dysfunction plays a central role in the pathogenesis of pulmonary arterial hypertension (PAH), promoting vasoconstriction, smooth muscle proliferation, and inflammation. This study sought to test the hypothesis that nebivolol, a β1-antagonist and β2,3-agonist, may improve PAH and

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