Skip to Content
Merck
All Photos(1)

Key Documents

G005743

HPLC Analysis of Steroids on Ascentis® Express F5

application for HPLC

Sign Into View Organizational & Contract Pricing
All Photos(1)

About This Item

technique(s)

HPLC: suitable

test parameters

column: Ascentis Express F5, 15 cm x 4.6 mm I.D., 2.7 μm particles (53591-U)
mobile phase: [A] water; [B] methanol; (50:50, A:B)
flow rate: 0.8 mL/min
pressure: 2393 psi (165 bar)
column temp.: 35 °C
detector: UV, 240 nm
injection: 5 μL
sample: 10 μg/mL of each in 90: 10, water: methanol

suitability

application for HPLC

application(s)

clinical

Analysis Note

Sample concentrations at 10 ug/mL in 90:10 water:methanol. Acetonitrile does not provide adequate selectivity on this phase. Mass spec was used for peak identification.

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

Analyte

Description
    Hydrocortisone
    Prednisolone
    Prednisone

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number

Sorry, we don't have COAs for this product available online at this time.

If you need assistance, please contact Customer Support.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service