G001580
HPLC Analysis of Insulin (Species Variants) on Discovery® BIO Wide Pore C5
application for HPLC
About This Item
technique(s)
HPLC: suitable
test parameters
column: Discovery BIO Wide Pore C5, 15 cm × 4.6 mm I.D., 5 μm particles (568422-U)
mobile phase: [A]: 71:29, (0.1% TFA in water):(0.1% TFA in acetonitrile);
[B]: 68:32, (0.1% TFA in water):(0.1% TFA in acetonitrile)
gradient: 0-100% B in 30 min
flow rate: 1 mL/min
column temp.: ambient
detector: UV, 215 nm
injection: 5 μL 5 μL in 0.1% TFA containing 5ug each of three insulin variants
suitability
application for HPLC
application(s)
life science and biopharma
pharmaceutical (small molecule)
Legal Information
analytical column
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