ZRB2373

Anti-Laminin-5 Antibody, clone 1D3 ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• Synonym(s): CSF 140 kDa subunit, Cell-scattering factor 140 kDa subunit, Epiligrin subunit gamma, Kalinin subunit gamma, Kalinin/nicein/epiligrin 100 kDa subunit, Ladsin 140 kDa subunit, Laminin B2t chain, Laminin subunit gamma-2, Laminin-5 subunit gamma, Large adhesive scatter factor 140 kDa subunit, Nicein subunit gamma
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: rabbit
• Quality Level: 200
• recombinant: expressed in HEK 293 cells
• conjugate: unconjugated
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 1D3, recombinant monoclonal
• description: 1D3 Clone
• product line: ZooMAb^® learn more
• form: lyophilized
• mol wt: calculated mol wt 130.98 kDa; observed mol wt ~140 kDa
• purified by: using Protein A
• species reactivity: human
• species reactivity (predicted by homology): monkey
• packaging: antibody small pack of 25 μL
• greener alternative product characteristics: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• enhanced validation: recombinant expression; Learn more about Antibody Enhanced Validation
• technique(s): immunocytochemistry: suitable; immunohistochemistry: suitable; western blot: suitable
• isotype: IgG
• epitope sequence: C-terminal half
• Protein ID accession no.: NP_005553
• UniProt accession no.: Q13753
• greener alternative category: Aligned
• shipped in: ambient
• storage temp.: 2-8°C
• Gene Information: human ... LAMC2(3918)

Description

General description

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry. This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Specificity

Clone 1D3 is a ZooMAb^® rabbit recombinant monoclonal antibody that specifically detects Laminin-5. It targets an epitope within 24 amino acids from the C-terminal half.

Immunogen

KLH-conjugated linear peptide corresponding to 24 amino acids from the C-terminal half of human Laminin subunit 2 (Laminin-5).

Application

Quality Control Testing

Evaluated by Western Blotting in A431 cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Laminin-5 in A431 cell lysate.

Tested Applications

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Laminin-5 in A431 cell membrane extract.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected Laminin-5 in Human placenta tissue sections.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Laminin-5 in A431 cells.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Target description

Laminin subunit gamma-2 (UniProt: Q13753; also known as Cell-scattering factor 140 kDa subunit, CSF 140 kDa subunit, Epiligrin subunit gamma, Kalinin subunit gamma, Kalinin/nicein/epiligrin 100 kDa subunit, Ladsin 140 kDa subunit, Laminin B2t chain, Laminin-5 subunit gamma, Large adhesive scatter factor 140 kDa subunit, Nicein subunit gamma) is encoded by the LAMC2 (also known as LAMB2T, LAMNB2) gene (Gene ID: 3918) in human. Laminin is a major basement membrane glycoprotein consisting of α, β, and γ chains that are bound to each other by disulfide bonds.  It is a key component of the anchoring complex connecting keratinocytes to the underlying dermis. Over 15 different isoforms have been reported in the laminin family that are expressed in a tissue-specific manner and play different role in each tissue. γ2, a subunit of Laminin-5 (also known as Laminin-332), is synthesized with a signal peptide (aa 1-21), which is subsequently cleaved off to generate the mature form. It is reported to be frequently over-expressed as a monomer or as the β3-γ2 heterodimer in invasive cancers and is regarded as an invasion marker. It has 8 EGF-like domains. The cell adhesion and motility activities of Laminin-5 are reported to be regulated by the proteolytic cleavage of the short arm of Laminin 2 chain by BMP-1 or MT1-MMP. This cleavage releases a 45 kDa N-terminal proteolytic fragment (γ2pf). The short arm and γ2pf can be further cleaved by serine proteases and by matrix metalloproteinases resulting in the release of small N-terminal fragments. γ2pf‐like fragments have been reported to be present in conditioned media of cultured cancer cells and in sera from human cancer subjects. This ZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Miyazaki, K., et al. (2016). Cancer Sci. 107(12); 1909-1918; Sato, H., et al. (2014). Cancer Sci. 105(2); 168-175; Schneider, H., et al. (2007). Eur. J. Cell Biol. 86(11-12); 701-717).

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

Reconstitution

300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable