ZRB2252

Anti-HLA-DPB1 Antibody, clone 1E16 ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• Synonym(s): HLA class II histocompatibility antigen DP(W4) beta chain, HLA class II histocompatibility antigen, DP beta 1 chain, MHC class II antigen DPB1
• UNSPSC Code: 12352203

Properties

• biological source: rabbit
• Quality Level: 200
• recombinant: expressed in HEK 293 cells
• conjugate: unconjugated
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 1E16, recombinant monoclonal
• description: recombinant, expressed in HEK 293 cells
• product line: ZooMAb^® learn more
• form: lyophilized
• purified by: using Protein A
• species reactivity: human
• packaging: antibody small pack of 25 μL
• greener alternative product characteristics: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• enhanced validation: recombinant expression; Learn more about Antibody Enhanced Validation
• technique(s): affinity binding assay: suitable; flow cytometry: suitable; immunocytochemistry: suitable; immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable; western blot: suitable
• isotype: IgG
• epitope sequence: Extracellular domain
• Protein ID accession no.: NP_002112
• UniProt accession no.: P04440
• greener alternative category: , Aligned
• shipped in: ambient
• storage temp.: 2-8°C
• Gene Information: human ... HLA-DPB1(3115)

Description

General description

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Specificity

Clone 1E16 is a ZooMAb^® rabbit recombinant monoclonal antibody that specifically detects MHC class II antigen DPB1 (HLA-DPB1). It targets an epitope within 18 amino acids from the extracellular domain.

Immunogen

KLH-conjugated linear peptide corresponding to 18 amino acids from the extracellular domain of human MHC class II antigen DPB1 (HLA-DPB1).

Application

Quality Control Testing

Evaluated by Western Blotting in Human tonsil tissue lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected HLA-DPB1 in Human tonsil tissue lysate.

Tested Applications

Flow Cytometry Analysis: 1 µg from a representative lot detected HLA-DPB1 in one million Human peripheral blood mononuclear cells (PBMC).

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected HLA-DPB1 in HEK 293 cells.

Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected HLA-DPB1 in human tonsil tissue sections.

Affinity Binding Assay: A representative lot of this antibody bound HLA-DPB1 peptide with a KD of 1.0 x 10-7 in an affinity binding assay.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Target description

HLA class II histocompatibility antigen, DP beta 1 chain (UniProt: P04440; also known as HLA class II histocompatibility antigen, DP(W4) beta chain, MHC class II antigen DPB1) is encoded by the HLA-DPB1 (also known as HLA-DP1B) gene (Gene ID: 3115) in human. HLA-DPB1 is a single-pass type I membrane protein that is synthesized with a signal peptide (aa 1-29), which is subsequently cleaved off to produce the mature form that contains an extracellular domain (aa 30-225), a transmembrane domain (aa 226-246), and a cytoplasmic domain (aa 227-258). It plays a central role in the immune system by presenting peptides derived from extracellular proteins. It is a MHC class II molecule that forms a heterodimer of α and β subunits. Its expression is observed in antigen presenting cells. It is reported to bind peptides derived from antigens that ACCESS^™ the endocytic route of antigen presenting cells (APC^™) and present them on the cell surface for recognition by the CD4 T-cells. Its peptide binding cleft accommodates peptides of 10-30 residues. Endocytosed exogenous antigens are readily available for presentation and compete with endogenous proteins on their way to degradation in lysosomes. Genetic variants in HLA-DPB1 are associated with increased risk of severe aplastic anemia. A mutation in HLA-DPB1 gene (HLA-DPB1Glu69) is observed in chronic beryllium disease. This ZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, Affinity^™, reproducibility, and stability over conventional monoclonals. (Ref.: McCanlies, EC., et al (2003). Am. J. Epidemiol. 157(5); 388-398).

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

Reconstitution

300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

ACCESS is a trademark of Millipore Corporation

Affinity is a trademark of Mine Safety Appliances Co.

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable