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Y0875

Sigma-Aldrich

Select Yeast Extract

for use in microbial growth medium

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About This Item

CAS Number:
8013-01-2
MDL number:
MFCD00132599
UNSPSC Code:
41106212
NACRES:
NA.85

grade

for molecular biology

sterility

non-sterile

form

powder

technique(s)

microbiological culture: suitable

pH

6.5-7.5(10% solution)

solubility

H2O: 100 mg/mL, clear to slightly hazy, yellow to brownish-yellow

application(s)

microbiology

storage temp.

room temp

suitability

nonselective for Escherichia coli
nonselective for coliforms

InChI

1S/C19H14O2/c20-18(21)11-17-15-8-4-3-7-14(15)16-10-9-12-5-1-2-6-13(12)19(16)17/h1-10,17H,11H2,(H,20,21)

InChI key

GQNBDGXKDJSVGQ-UHFFFAOYSA-N

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General description

Yeast extract is the water-soluble portion of autolyzed yeast, often used in culture media. Yeast extract contains a mixture of amino acids, peptides, water soluble vitamins and carbohydrates.
Yeast extract is used in all solid media, on which stock cultures are maintained, due to its ability in promoting growth of most bacterial contaminants, thereby making them visible to identify. Yeast extract does not enhance the growth of wild-type cells but essential for auxotrophic mutants.

Application

Select Yeast Extract has been used:
  • as a supplement in the minimal sporulating medium (MSL) for the secretory production of human tumor necrosis factor-α (TNFα) using the carboxypeptidase Y (Cpy) signal peptide.
  • in the isolation of halophilic archaea.
  • as a component of microbial growth medium for cultivation of marine bacterial strain Zobellia uliginosa.

    Suitable for use as nutritional source in microbial media.
Yeast extract is used as a bacteriological media component for a variety of microorganisms and molecular genetics applications.

Other Notes

Water soluble portion of autolyzed yeast with intact B-complex vitamins. Yeast extract is a mixture of amino acids, peptides, water soluble vitamins and carbohydrates and can be used as additive for culture media.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Chlamydomonas in the Laboratory
The Chlamydomonas Sourcebook (2009)
Characterization and antimicrobial potential of extremely halophilic archaea isolated from hypersaline environments of the Algerian Sahara
Quadri I, et al.
Microbiological research, 186, 119-131 (2016)
Søren Kjaerulff et al.
Biochemical and biophysical research communications, 336(3), 974-982 (2005-09-15)
In the fission yeast Schizosaccharomyces pombe, there are relatively few signal peptides available and most reports of their activity have not been comparative. Using sequence information from the S. pombe genome database we have identified three putative signal peptides, designated
Comparison of different signal peptides for secretion of heterologous proteins in fission yeast
Kjaerulff S and Jensen MR
Biochemical and Biophysical Research Communications, 336(3), 974-982 (2005)
Kim M Handley et al.
International journal of systematic and evolutionary microbiology, 59(Pt 4), 886-892 (2009-03-31)
A Gram-negative, arsenate-respiring and arsenite-oxidizing marine bacterium, NKSG1(T), was isolated from hydrothermal sediment at Santorini, Greece. Strain NKSG1(T) was a facultatively anaerobic, motile, non-spore-forming, rod-shaped bacterium. Growth occurred optimally at 35-40 degrees C, between pH 5.5 and 9.0 and with
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