SRP3271

GM-CSF rat

Animal-component free, recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC)

• Synonym(s): CSF-3, GM-CSF?, MGI-1G, pluripoietin
• UNSPSC Code: 12352202

Properties

• biological source: rat
• recombinant: expressed in E. coli
• Assay: ≥98% (HPLC); ≥98% (SDS-PAGE)
• form: lyophilized
• potency: ≥0.01 ng/mL
• mol wt: 14.5 kDa
• packaging: pkg of 20 μg
• impurities: <0.1 EU/μg endotoxin, tested
• color: white to off-white
• UniProt accession no.: P01587
• shipped in: wet ice
• storage temp.: −20°C
• Gene Information: rat ... CSF2(12981)

Description

General description

It is produced in by endothelial cells, monocytes, fibroblasts and T-lymphocytes. GM-CSF inhibits neutrophil migration and enhances the functional activity of the mature end-cells. The human and murine molecules are species-specific and exhibit no cross-species reactivity.

Biochem/physiol Actions

GM-CSF is a hematopoietic growth factor that stimulates the development of neutrophils and macrophages and promotes the proliferation and development of early erythroid megakaryocytic and eosinophilic progenitor cells. Recombinant murine GM-CSF is a 14.5 kDa globular protein consisting of 128 amino acids residues.

Physical form

Lyophilized from 10 mM Tris, pH 8.5

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable