SAB5801006

Alpaca Anti-Mouse IgG2a(Fcγ) single domain Antibody

recombinant Alpaca(VHH) Nano antibody

• Synonym(s): Recombinant Alpaca single variable domain secondary Nanobody
• UNSPSC Code: 12352203

Properties

• biological source: alpaca
• recombinant: expressed in E. coli
• conjugate: unconjugated (6*his tag and one cys were added at the C terminal of the VHH)
• antibody form: affinity purified immunoglobulin
• antibody product type: secondary antibodies
• clone: recombinant monoclonal
• form: lyophilized solid
• mol wt: 14 kDa
• species reactivity: Alpaca, mouse
• technique(s): ELISA: 1:10000-1:50000; western blot: 1:10000-1:50000
• size: 0.1 mL
• color: colorless
• isotype: VHH
• shipped in: wet ice
• storage temp.: 2-8°C

Description

General description

Alpaca Anti-Mouse IgG2a(Fcγ) single domain Antibody is designed to specifically detect mouse IgG2a Fcγ fragment. It is a monovalent, single domain, recombinant antibody derived from the variable region of heavy chain of Alpaca pacous. Based on immunoelectrophoresis and/or ELISA, Alpaca Anti-Mouse IgG2a(Fcγ) single domain Antibody reacts with the Fc fragment of mouse IgG2a selectively but not with other mouse IgG subclasses, mouse IgM, or the Fab portion of mouse immunoglobulins.
Most monoclonal antibodies are generated in mouse. There are five antibody isotypes (IgA, IgD, IgE, IgG, and IgM) from mouse. Each isotype has a different heavy chain. Mouse IgG constitutes 75% of serum immunoglobulins, and IgG is the predominant form of first antibody produced from mouse. Mouse IgG consists of five subclasses-IgG1, IgG2a, IgG2b, IgG2c(inbred mouse strains with the Igh1-b allele have IgG2c isotype instead of IgG2a), IgG3. They are highly homologous and differ mainly in the hinge region. The whole IgG molecule possesses both the Fc region and the Fab region, which possessing the epitope-recognition site. The IgG contains two heavy and light chains, and the heavy chain is about 50 KD and the light chain is about 25 KD. The common IgG is monomeric with a molecular weight of approximately 150 kD.
VHH are single-domain antibodies derived from the variable regions of heavy chain of Camelidae immunoglobulin. The size of VHH is extremely small(<15KDa) compared to other forms of antibody fragment, which significantly increase the permeability of VHH. Thus VHH is considered of great value for research, diagnostics and therapeutics.

Specificity

Mouse IgG2a (Fcγ fragment specific)
Isotype: VHH domain of alpaca IgG2b/2c

Immunogen

Recombinant Alpaca single variable domain secondary nano antibody

Packaging

100 μg in Glass bottle

Physical form

Lyophilized from 10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose

Reconstitution

Add 100uL dH2O or 100uL dH2O with 50% Glycerol to a final concentration of 1mg/mL

Storage and Stability

After reconstitution, store at –20 °C(Avoid freeze / thaw cycles). Stable for 12 months at -20°C

Other Notes

No cross-reactivity with mouse IgG1/2b/3, mouse IgM, rabbit, human, cynomolgus, rat, goat IgG

Safety Information

• Pictograms: GHS07
• Signal Word: Warning
• Hazard Statements: H317 - H412
• Precautionary Statements: P261 - P272 - P273 - P280 - P302 + P352 - P333 + P313
• Hazard Classifications: Aquatic Chronic 3 - Skin Sens. 1
• Storage Class Code: 13 - Non Combustible Solids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable