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Key Documents

SAB4700102

Sigma-Aldrich

Monoclonal Anti-CD14, low endotoxin antibody produced in mouse

clone MEM-18, purified immunoglobulin, buffered aqueous solution

Synonym(s):

Anti-LPS-receptor

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About This Item

UNSPSC Code:
12352203

biological source

mouse

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

MEM-18, monoclonal

form

buffered aqueous solution

species reactivity

nonhuman primates, human

concentration

1 mg/mL

technique(s)

flow cytometry: suitable

isotype

IgG1

NCBI accession no.

NM_000591.3

UniProt accession no.

P08571

shipped in

wet ice

storage temp.

2-8°C

target post-translational modification

unmodified

Gene Information

human ... CD14(929)

Related Categories

General description

The antibody MEM-18 reacts with CD14, a 53-55 kDa GPI (glycosylphosphatidylinositol)-linked membrane glycoprotein expressed on monocytes, macrophages and weakly on granulocytes; also expressed by most tissue macrophages. In human, the epitope recognized by MEM-18 is located between amino acids 57-64.

Immunogen

A crude mixture of human urinary proteins precipitated by ammonium sulphate from the urine of a patient suffering from proteinuria

Application

The reagent is designed for Flow Cytometry analysis. Suggested working dilution for Flow Cytometry is 4 μg/mL of sample. Indicated dilution is recommended starting point for use of this product. Working concentrations should be determined by the investigator.

Features and Benefits

Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.

Physical form

Solution in azide free phosphate buffered saline, pH 7.4; 0.2 um filter sterilized. Endotoxin level is less than 10 EU/mg of the protein, as determined by the LAL test.

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Storage Class Code

10 - Combustible liquids

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Catherine E Angel et al.
International immunology, 19(11), 1271-1279 (2007-09-07)
We recently demonstrated that three antigen-presenting cell (APC) subsets exist in the healthy human dermis, CD14(+) and CD1a(+) dermal APCs and migratory dermal Langerhans cells. Here, we extend these findings by defining CD208 as an exclusive marker of migratory dermal

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