SAB4504210

Anti-phospho-Smad3 (pSer²⁰⁴) antibody produced in rabbit

affinity isolated antibody

• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: rabbit
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: antigen 48 kDa
• species reactivity: mouse, human, rat
• concentration: ~1 mg/mL
• technique(s): ELISA: 1:20000; western blot: 1:500-1:1000
• NCBI accession no.: P84022
• UniProt accession no.: P84022
• application(s): research pathology
• shipped in: wet ice
• storage temp.: −20°C
• target post-translational modification: phosphorylation (pSer204)
• Gene Information: human ... SMAD3(4088)

Description

General description

The SMAD3 (mothers against decapentaplegic homolog 3) gene is mapped to human chromosome 15q22.33. SMAD proteins contains three main domains : MH1 (MAD homology 1) - amino terminal domain (highly conserved), MH2 - carboxy terminal domain and a linker domain between the two domains. The MH1 domain of Smad3 serves as a DNA binding motif.(8)

Immunogen

The antiserum was produced against synthesized peptide derived from human Smad3 around the phosphorylation site of Ser204.

Immunogen Range: 170-219

Application

Anti-phospho-Smad3 (pSer204) antibody produced in rabbit has been used in western blot analysis.(7)

Biochem/physiol Actions

Smad3 (mothers against decapentaplegic homolog 3) protein identifies tandem repeats of the palindromic sequence GTCTAGAC, which is part of TGF-β (transforming growth factor β) signaling promoter region. Smad3 is associated with TGF-β (transforming growth factor beta) signaling pathway, a cellular process regulating the homeostasis, development, and repair of cartilage. It is known to induce extracellular matrix production. Smad proteins are responsible for the transduction of signals from cell surface to the nucleus. Phosphorylation of Smad3 controls tumor progression, inflammation, fibrosis, obesity and diabetes. Smad3 expression might be associated with the pathogenesis of osteoarthritis.

Features and Benefits

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Physical form

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable