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SAB4200782

Sigma-Aldrich

Anti-Tenascin antibody, Mouse monoclonal

clone BC-24, purified from hybridoma cell culture

Synonym(s):

Anti-Cytotactin, Anti-GMEM, Anti-GP 150-225, Anti-Glioma-associated-extracellular matrix antigen, Anti-Hexabrachion, Anti-JI, Anti-Myotendinous antigen, Anti-Neuronectin, Anti-TN, Anti-Tenascin-C (TN-C)

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

biological source

mouse

Quality Level

200

antibody form

purified from hybridoma cell culture

antibody product type

primary antibodies

clone

BC-24, monoclonal

form

buffered aqueous solution

species reactivity

human

concentration

~1.0 mg/mL

technique(s)

ELISA: suitable
flow cytometry: suitable
immunoblotting: suitable
immunohistochemistry: 5-10 μg/mL using pronase-retrieved formalin-fixed, paraffin-embedded human tonsil sections

isotype

IgG1

UniProt accession no.

P24821

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... TNC(3371)

Related Categories

General description

Monoclonal Anti-Tenascin (mouse IgG1 isotype) is derived from the BC-24 hybridoma, produced by the fusion of mouse myeloma cells and splenocytes from a mouse immunized with human tenascin. Tenascin-C (TN-C) is also known as Hexabrachion, Cytotactin, Neuronectin (NEC1). It is a high molecular mass extracellular matrix glycoprotein. Human tenascin is a disulfide-linked hexamer composed of 3 subunits of 190, 200, and 220 kDa.

Immunogen

Human tenascin

Application

Anti-Tenascin antibody has been used:
  • in immunoblotting
  • in Immunohistochemistry
  • in flow cytometry
  • in enzyme linked immunosorbent assay(ELISA)
  • for blocking tenascin-C proliferating activity

Biochem/physiol Actions

Tenascin-C (TN-C) functions in cell adhesion, fibroblast migration, and other processes related to tissue remodeling and wound healing. It has been proposed that actively growing, migrating and differentiating epithelial sheets can produce factors such as (Transforming growth factor beta) TGF-β to stimulate tenascin expression. Neo-expression or increased expression of tenascin has been found in the stroma of various tumors and during normal tissue repair. Intracytoplasmic tenascin immunoreactivity has been detected in malignant melanomas and in lung carcinomas, and it serves as a marker of stromal element proliferation in invasive breast carcinomas. High-molecular mass tenascin isoform plays a role in generating a permissive environment for proliferation, invasion, and metastasis of neoplastic epithelial cells. Human tenascin contains an Arg-Gly-Asp (RGD) sequence which may function in cell adhesion and mediates cell attachment through an RGD-dependent integrin receptor.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide as a preservative.

Other Notes

This product is for R&D use only, not for drug, household, or other uses.

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Storage Class Code

10 - Combustible liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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The role of tenascin-C in tissue injury and tumorigenesis
Midwood K S, et al.
Journal of Cell Communication and Signaling, 3(3-4), 287-310 (2009)
Tenascin-C modulates tumor stroma and monocyte/macrophage recruitment but not tumor growth or metastasis in a mouse strain with spontaneous mammary cancer
Talts J F, et al.
Journal of Cell Science, 112(12), 1855-1864 (1999)
Christopher R Silvers et al.
British journal of cancer, 125(10), 1399-1407 (2021-09-27)
Markers of stromal activation at future metastatic sites may have prognostic value and may allow clinicians to identify and abolish the pre-metastatic niche to prevent metastasis. In this study, we evaluate tenascin-C as a marker of pre-metastatic niche formation in
L Borsi et al.
International journal of cancer, 52(5), 688-692 (1992-11-11)
Functionally different tenascin (TN) isoforms, containing varying numbers of a 91 amino-acid motif resembling the fibronectin type-III homology repeat, may be generated by alternative splicing of the TN primary transcript. In fact, only the TN isoform containing the alternatively spliced
Gregory S Schultz et al.
Wound repair and regeneration : official publication of the Wound Healing Society [and] the European Tissue Repair Society, 17(2), 153-162 (2009-03-27)
Dynamic interactions between growth factors and extracellular matrix (ECM) are integral to wound healing. These interactions take several forms that may be categorized as direct or indirect. The ECM can directly bind to and release certain growth factors (e.g., heparan
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