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Key Documents

SAB2702295

Sigma-Aldrich

Monoclonal Anti-mCherry antibody produced in mouse

clone GT844, affinity isolated antibody

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

biological source

mouse

Quality Level

100

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

GT844, monoclonal

form

buffered aqueous solution

mol wt

27kDa

concentration

1mg/mL

technique(s)

immunofluorescence: 1:100-1:1,000
western blot: 1:500-1:3,000

isotype

IgG2a

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

unmodified

General description

The acceptor mCherry is a monomeric, red fluorescent protein obtained from the stony-coral Discosoma protein DsRED. It is expressed in the cytoplasm, has a high photostability and is unaffected by photobleaching. mCherry acts as a good FRET (fluorescence resonance energy transfer) acceptor. mCherry allows rapid fluorescence detection after a particular gene is expressed.

Immunogen

Full length mCherry recombinant protein

Application

Suggested starting dilutions are as follows: WB: 1:500-1:3000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Features and Benefits

Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.

Other Notes

Purification: Affinity purified by Protein G

Physical form

Phosphate-buffered saline, no preservative added.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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In vitro screening test using Leishmania promastigotes stably expressing mCherry protein.
Vacchina P and Morales MA
Antimicrobial Agents and Chemotherapy, 58(3), 1825-1828 (2014)
Quantitative FRET Analysis With the E0GFP?mCherry Fluorescent Protein Pair.
Albertazzi L, et al.
Photochemistry and Photobiology, 85(1), 287-297 (2009)

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