M7528

Medium 199

HEPES modification, with Earle′s salts, and sodium bicarbonate, without ʟ-glutamine, liquid, sterile-filtered, suitable for cell culture

• Synonym(s): Cell culture medium, M199 Medium, TCM 199
• UNSPSC Code: 12352207
• NACRES: NA.75

Properties

• product name: Medium 199, HEPES Modification, with Earle′s salts, 25 mM HEPES and sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture
• sterility: sterile-filtered
• form: liquid
• technique(s): cell culture | mammalian: suitable
• impurities: endotoxin, tested
• pH: >7.2
• components: L-glutamine: no; sodium pyruvate: no; HEPES: 25 mM; phenol red: yes; NaHCO₃: yes; Earle’s salts (5% CO₂): yes
• shipped in: ambient
• storage temp.: 2-8°C

Description

General description

Medium 199 is a combination of vitamins, amino acids, and other factors that were originally developed as a completely defined media formulation for the culture of primary explants. This medium, when properly supplemented has broad applicability, particularly for non-transformed cells. Medium 199 is widely used for vaccine production, in vitro cultivation of primary pancreatic explants, and lens tissues.

Reconstitution

Supplement with 0.1 g/L L-glutamine.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable