I4640
Human IgG4 Kappa, Recombinant Protein
purified immunoglobulin, buffered aqueous solution
Synonym(s):
Human IgG4 Protein
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About This Item
biological source
human
Quality Level
conjugate
unconjugated
antibody form
purified immunoglobulin
Assay
>95% (SDS-PAGE)
form
buffered aqueous solution
shipped in
dry ice
storage temp.
−20°C
General description
IgG antibody subtype is the most abundant serum immunoglobulin of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to the fetus through the placenta and is vital for immune defense of the neonate against infections. IgG4, κ recombinant protein is obtained by expression of a phage library in a human cell line and purified by affinity chromatography.
Application
The purified IgG4, κ may be used as an immunoglobulin calibrator, reference antigen, blocking agent, or coating protein in a variety of immunoassays including ELISA, dot blot immunoblotting, Western immunoblotting, immunodiffusion, immunoelectrophoresis, hemagglutination, and cell-binding assays.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4.
Analysis Note
The purity and identity are determined by immunoelectrophoresis, indirect ELISA, and SDS-PAGE.
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
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