H8162

HIS-Select^® Cobalt Affinity Gel

(1:1 suspension in a 30% ethanol solution)

• Synonym(s): cobalt affinity gel, cobalt charged agarose
• UNSPSC Code: 12352200
• NACRES: NA.56

Properties

• Quality Level: 200
• form: (1:1 suspension in a 30% ethanol solution)
• shelf life: 1 yr (when stored properly.)
• technique(s): protein purification: suitable
• matrix: 6% Beaded Agarose
• capacity: >15 mg/mL, agarose binding capacity (protein)(with an approx. 30 kDa protein)
• storage temp.: 2-8°C

Description

General description

The HIS-Select Cobalt Affinity Gel is a quadridentate chelate on beaded agarose that is charged with cobalt. The gel is designed to specifically bind histidine-containing proteins / His-tag proteins. It is selective for recombinant proteins with histidine tags and exhibits low non-specific binding of other proteins.
HIS-Select Cobalt Affinity Gel is an immobilized metal-ion affinity chromatography (IMAC) product. The matrix for this affinity gel is 6% beaded agarose. The selectivity can be modulated with the inclusion of imidazole during chromatography. HIS-Select Cobalt Affinity Gel is durable and can capture recombinant proteins with histidine tags at a high flow rate. Recombinant proteins with His tags are bound using native, denaturing, or mild reducing conditions. The capacity of this affinity gel is typically >15 mg/mL of packed gel, determined with a His-tagged protein of molecular mass ~30 kDa.

Application

HIS-Select Cobalt Affinity Gel allows for high purity, low non-specific binding, and high binding capacity of protein. HIS-Select Cobalt Affinity Gel also works well for purification of histidine-tagged proteins / His-tag proteins in native, denaturing, or mild reducing conditions. HIS-Select^™ Cobalt Affinity Gel has been used to purify His-tagged proteins.

Physical form

1:1 suspension in a 30% ethanol solution

Legal Information

HIS-Select is a registered trademark of Merck KGaA, Darmstadt, Germany

HiScreen is a trademark of Cytiva