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Key Documents

GE17-0230-01

SP-Sephadex® C-25

Cytiva 17-0230-01, pack of 100 g

Synonym(s):

Affinity Gel, Sephadex C-25

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About This Item

UNSPSC Code:
47101511
NACRES:
NA.56

feature

autoclavable (30 min at 121oC in 0.1M sodium chloride)

packaging

pack of 100 g

manufacturer/tradename

Cytiva 17-0230-01

matrix

cross-linked dextran base material (Sephadex)

matrix active group

sulphopropyl functional group

particle size

40-100 μm (dry)

operating pH

2-13

capacity

2-2.6 mmol/g ion exchange capacity

separation technique

cation exchange

storage temp.

room temp

General description

Sephadex® ion exchangers are produced by introducing functional groups onto Sephadex, a cross-linked Dextran matrix. These groups are attached to glucose units in the matrix by stable ether linkages.
lgG (Mr 160 000)- 1.1 mg/ml drained medium), Bovine COHb (Mr 69 000)- 70 mg/ml drained medium), Ribonuclease (Mr 13 700)- 230 mg/ml drained medium)

Application

The excellent chemical and physical stability plus the ease of packing and cleaning have made Sephadex® A-25 and C-25 types popular choices for a wide range of applications. They are used especially for separating small Proteins, peptides and carbohydrates because of the Low non-specific adsorption of these types of biomolecules.

Storage and Stability

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Legal Information

Sephadex is a registered trademark of Cytiva

Storage Class Code

13 - Non Combustible Solids

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Protocols

Performing a Separation with Sephadex®

This page shows how to perform a separation with a Sephadex column from GE Healthcare which are Size Exclusion Chromatography media consisting of an epichlorohydrin cross-linked dextran.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service