Skip to Content
Merck
All Photos(3)

Documents

F3520

Sigma-Aldrich

Anti-von Willebrand Factor antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

Synonym(s):

Von Willebrand Factor Antibody, Von Willebrand Factor Antibody - Anti-von Willebrand Factor antibody produced in rabbit, Anti-Factor VIII-Related Antigen

Sign Into View Organizational & Contract Pricing
All Photos(3)

About This Item

MDL number:
MFCD00162556
UNSPSC Code:
12352203
NACRES:
NA.41

biological source

rabbit

Quality Level

300

conjugate

unconjugated

antibody form

IgG fraction of antiserum

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

species reactivity

human

packaging

antibody small pack of 25 μL

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:1,000 using human tissue (Indirect Immunoperoxidase Labeling)
indirect immunofluorescence: 1:200 using formalin-fixed, paraffin-embedded sections of human tissue

UniProt accession no.

P04275

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... VWF(7450)

Related Categories

General description

Anti-von Willebrand Factor is produced in rabbit using purified von Willebrand factor (factor VIII R:Ag) as the immunogen.
Human von Willebrand factor (factor VIII R:Ag) is a 270 kDa multimeric plasma gylcoprotein. The von Willebrand factor has functional binding domains to platelet glycoprotein Ib, glycoprotein IIb/IIIa, collagen and heparin. The factor is synthesized by endothelial cells and is also present in platelets and megakaryocytes.

Specificity

The antibody specifically reacts with the cytoplasm of human endothelial cells from normal and neoplastic blood and from lymphatic vessels. It also reacts with human endocardium, platelets and megakaryocytes. It may be used for immunohistological studies of the vascular structures in frozen tissue preparations and enzymatically pre-digested, formalin, methacarn, Bouin′s or ethanol-fixed and paraffin-embedded tissue sections.

Immunogen

von Willebrand Factor (Factor VIII-related antigen) from human plasma.

Application

Anti-von Willebrand Factor antibody produced in rabbit has been used for:
  • western blot analysis and immunostaining
  • immunocytochemistry
  • immunohistochemistry
  • indirect immunofluorescence

Biochem/physiol Actions

It mediates platelet adhesion to injured vessel walls and serves as a carrier and stabilizer for coagulation factor VIII.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide

Preparation Note

Whole antiserum is purified to provide an IgG fraction of antiserum.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Not finding the right product?  

Try our Product Selector Tool.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

J Marc Simard et al.
Stroke, 41(3), 531-537 (2010-01-23)
Treating patients with malignant cerebral infarctions remains a major unsolved problem in medicine. Decompressive craniectomy (DC) improves the bleak outlook but is suboptimal. Using a rat model of severe ischemia/reperfusion with very high mortality due to malignant cerebral edema, we
Mas receptor is involved in the estrogen-receptor induced nitric oxide-dependent vasorelaxation
Sobrino A, et al.
Biochemical Pharmacology, 129, 67-72 (2017)
Gerit Moser et al.
Histochemistry and cell biology, 147(3), 353-366 (2016-10-25)
During the first trimester of pregnancy, extravillous trophoblasts (EVTs) invade into the decidual interstitium to the first third of the myometrium, thereby anchoring the placenta to the uterus. They also follow the endovascular and endoglandular route of invasion; plug, line
Babett Bartling et al.
Molecular medicine (Cambridge, Mass.), 17(9-10), 980-989 (2011-06-02)
The plasma fluorescence related to the standard fluorescence of advanced glycation end products (AGEs) is a simple measurable blood parameter for distinct diseases but its importance in human cancer, including non-small cell lung carcinoma (NSCLC), is unknown. Plasma samples of
MicroRNA as crucial regulators of gene expression in estradiol-treated human endothelial cells
Vidal-Gomez X, et al.
Cellular Physiology and Biochemistry, 45(5), 1878-1892 (2018)
View All Related Papers

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service