EL4 Cell Line murine

85023105, mouse blood, Lymphoblast

• UNSPSC Code: 41106514

Properties

• product name: EL4 Cell Line murine, 85023105, Lymphoblast from blood
• biological source: mouse blood
• growth mode: Suspension
• karyotype: 2n = 39
• morphology: Lymphoblast
• products: A surface antigen induced by leukaemia type G virus; H-2b and Thy-1.2 antigens; Interleukin 4 (IL-4), H-2b, Thy1.2
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): cancer
• shipped in: dry ice
• storage temp.: −196°C

Description

Cell Line Origin

Mouse ascites lymphoma lymphoblast

Cell Line Description

Established from a lymphoma induced in a C57BL/6N mouse by 9,10-dimethyl-1,2-benzanthracene. The cells have been reported to produce low levels of type G (Gross) virus antigen histone-2b (H-2b), interleukin-4 (IL-4) and Thy-1.2 antigen. The cell line is resistant to cortisol and dexamethasone and is sensitive to polyhydroxyalkanoates (PHAs).

Application

EL4 Cell Line murine has been used to induce tumor in mice and to assess the extravasation of tumor necrosis factor (TNF)-induced Evans blue dye and E1- and E3-deleted Ad vectors carrying firefly luciferase reporter gene under cytomegalovirus promoter (AdLuc). It has also been used as a constituent of bone marrow-derived macrophage (BMDM)-polarizing media to polarize BMDM and assess its tumoricidal activity.

Tumourigenicity studies

Culture Medium

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Maintain cultures between 3-9x100,000 cells/ml; 5% CO₂; 37°C. Cell growth after resuscitation is slow. Subculture once exponential growth is established.

Other Notes

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