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biological source
Mouse x Mouse Hybridoma
clone
TAT-1
ImmunoDon
Mouse spleen
Myeloma
Sp2-0/Ag
Reactivity
Anti-α-Tubulin
technique(s)
cell culture | mammalian: suitable
isotype
IgG12b + IgG2a
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
Mouse x Mouse hybridoma
Cell Line Description
TAT-1 was developed by immunizing mice with extracted T. brucei brucei cytoskeleton and fusing the mouse spleen cells with the myeloma Sp2-0/Ag. This cell line has been cloned many times and the mixed subclass is now typical of the cloned line.
Immunogen
T. brucei brucei cytoskeleton (detergent extracted)
Application
Detection of α tubulin.
Culture Medium
Subculture Routine
Maintain cultures between 1-9 x 100,000 cells/ml; 5% CO2; 37°C
Other Notes
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Certificates of Analysis (COA)
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