C-12735
Human White Preadipocytes (HWP-c)
Subcutaneous, 1,000,000 cryopreserved cells
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About This Item
UNSPSC Code:
41106514
NACRES:
NA.81
biological source
human adipose tissue (subcutaneous)
form
liquid
packaging
pkg of 1,000,000 cells
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
General description
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Application
Primary Human White Preadipocytes (HWP) are isolated from adult subcutaneous adipose tissue from different locations (lot specific source information is available on request) and provided in a cryopreserved format. Adipose tissue is crucial in energy storage and metabolic homeostasis. An increase in adipose tissue results either from an enlargement of mature adipocytes or from the differentiation of adipocyte precursor cells (preadipocytes) into new mature adipocytes. These preadipocytes are already present in adipose tissues. They can proliferate throughout adult life and replace the cells that have differentiated into mature adipocytes. Since this differentiation process is controlled by a variety of growth factors and hormones, preadipocytes are suitable for the investigation of the physiological mechanisms controlling proliferation, differentiation, and function of adipose tissue.
Quality
Rigid quality control tests are performed for each lot of PromoCell Human White Preadipocytes. They are tested for cell morphology, adherence rate, and cell viability. Growth performance is tested through multiple passages up to 10 population doublings (PD) under culture conditions without antibiotics and antimycotics. Furthermore, each lot of PromoCell Human White Preadipocytes is extensively tested for its capacity to differentiate into mature adipocytes. In addition, all cells have been tested for the absence of HIV-1, HIV-2, HBV, HCV, HTLV-1, HTLV-2 and microbial contaminants (fungi, bacteria, and mycoplasma).
Warning
Although tested negative for HIV-1, HIV-2, HBV, HCV, HTLV-1 and HTLV-2, the cells – like all products of human origin – should be handled as potentially infectious. No test procedure can completely guarantee the absence of infectious agents.
Subculture Routine
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Other Notes
Recommended Plating Density: 5000 cells per cm2
Passage After Thawing: P2
Tested Markers: Differentiation tested
Population Doublings: >10
Passage After Thawing: P2
Tested Markers: Differentiation tested
Population Doublings: >10
Recommended products
Recommended Primary Cell Culture Media:Link
Recommended Primary Cell Culture Media:Link
Disclaimer
RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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