47614

Fluram

BioReagent, suitable for fluorescence, ≥99.0% (UV)

• Synonym(s): Fluorescamine, 4-Phenylspiro-[furan-2(3H),1-phthalan]-3,3′-dione
• Empirical Formula (Hill Notation): C₁₇H₁₀O₄
• CAS Number: 38183-12-9
• Molecular Weight: 278.26
• Beilstein: 921143
• EC Number: 253-814-5
• MDL number: MFCD00005928
• UNSPSC Code: 12352200
• PubChem Substance ID: 329757329

Properties

• product line: BioReagent
• Assay: ≥99.0% (UV)
• mp: 153-157 °C (lit.); 153-157 °C
• solubility: acetonitrile: soluble; ethanol: soluble
• fluorescence: λ_ex 234 nm; λ_ex 390 nm; λ_em 480 nm in 0.5 M borate pH 8.5 (after derivatization with L-leucine)
• suitability: suitable for fluorescence
• SMILES string: O=C1OC2(OC=C(C2=O)c3ccccc3)c4ccccc14
• InChI: 1S/C17H10O4/c18-15-13(11-6-2-1-3-7-11)10-20-17(15)14-9-5-4-8-12(14)16(19)21-17/h1-10H
• InChI key: ZFKJVJIDPQDDFY-UHFFFAOYSA-N

Description

Application

Fluram (fluorescamine) is a non-fluorescent reagent that reacts readily under mild conditions with primary amines in amino acids and peptides and other molecules to form stable, highly fluorescent compounds. Fluorescamine is useful for the fluorometric assay of amino acids, protein, and proteolytic enzymes and as a pre-column derivatization reagent. It effectively blocks newly generated amino termini in protein sequence analyses.

Non-fluorescent reagent that reacts readily under mild conditions with primary amines in amino acids and peptides to form stable, highly fluorescent compounds. Low background due to hydrolysis. Useful for the fluorometric assay of amino acids, protein, and proteolytic enzymes. Effectively blocks newly generated amino termini in protein sequence analyses.

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

Legal Information

Safety Information

• Storage Class Code: 13 - Non Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves