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Key Documents

S9763

Sigma-Aldrich

Sodium phosphate dibasic

ACS reagent, ≥99.0%

Synonym(s):

sec-Sodium phosphate, Disodium hydrogen phosphate, Disodium phosphate, Sodium hydrogenphosphate

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About This Item

Linear Formula:
Na2HPO4
CAS Number:
Molecular Weight:
141.96
EC Number:
MDL number:
UNSPSC Code:
12352302
eCl@ss:
38070211
PubChem Substance ID:
NACRES:
NA.21

grade

ACS reagent

Quality Level

Agency

suitable for SM 5210

Assay

≥99.0%

form

powder

impurities

≤0.002% Chloride (Cl)
≤0.01% Insoluble matter

loss

≤0.2% loss on drying, 105°C

pH

8.7-9.3 (25 °C, 5% in solution)

pKa (25 °C)

(1) 2.15, (2) 6.82, (3) 12.38 (phosphoric acid)

solubility

water: 5% at 25 °C, clear

anion traces

sulfate (SO42-): ≤0.005%

cation traces

Fe: ≤0.002%
heavy metals: ≤0.001% (by ICP-OES)

SMILES string

[Na+].[Na+].[H]OP([O-])([O-])=O

InChI

1S/2Na.H3O4P/c;;1-5(2,3)4/h;;(H3,1,2,3,4)/q2*+1;/p-2

InChI key

BNIILDVGGAEEIG-UHFFFAOYSA-L

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General description

Sodium phosphate dibasic in combination with sodium phosphate monobasic is used in the preparation of oral sodium phosphate solution (OSPS), which is a purgative used for bowel cleansing before colonoscopy.

Application

Sodium phosphate dibasic has been used in:
  • the preparation of phosphate buffer saline solution for perfusion procedure
  • western blot
  • indirect enzyme linked immunosorbent assay

Legal Information

Redi-Dri is a trademark of Sigma-Aldrich Co. LLC

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

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Glomerular calcification induced by bolus injection with dibasic sodium phosphate solution in Sprague-Dawley rats
Tsuchiya N, et al.
Toxicologic Pathology, 408-412 (2004)
Peri H Tate et al.
Methods (San Diego, Calif.), 53(4), 331-338 (2011-02-04)
The EUCOMM and KOMP programs have generated targeted conditional alleles in mouse embryonic stem cells for nearly 10,000 genes. The availability of these stem cell resources will greatly accelerate the functional analysis of genes in mice and in cultured cells.
Karsten Krug et al.
Cell, 183(5), 1436-1456 (2020-11-20)
The integration of mass spectrometry-based proteomics with next-generation DNA and RNA sequencing profiles tumors more comprehensively. Here this "proteogenomics" approach was applied to 122 treatment-naive primary breast cancers accrued to preserve post-translational modifications, including protein phosphorylation and acetylation. Proteogenomics challenged
Samuel Adler et al.
Gastrointestinal endoscopy, 76(6), 1170-1174 (2012-10-03)
Colon capsule endoscopy (CCE) is a noninvasive technique for the detection of colorectal lesions. However, for CCE to be offered as an out-of-clinic procedure, the system needs to automatically alert the patient when to ingest the laxative (booster). We tested
Megan A Rees et al.
Journal of proteome research, 14(1), 120-132 (2014-10-21)
Interactions between a host and a bacterial pathogen are mediated by cross-talk between molecules present on, or secreted by, pathogens and host binding-molecules. Identifying proteins involved at this interface would provide substantial insights into this interaction. Although numerous studies have

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