75554

Aminopeptidase Test

suitable for microbiology

• UNSPSC Code: 41171621
• NACRES: NA.85

Properties

• product line: BioChemika
• Quality Level: 100
• shelf life: limited shelf life, expiry date on the label
• technique(s): microbe id | specific enzyme detection: suitable
• antibiotic activity spectrum: Gram-negative bacteria
• application(s): agriculture; clinical testing; environmental; food and beverages; pharmaceutical; microbiology
• storage temp.: 2-8°C
• suitability: bacteria

Description

General description

Aminopeptidase Test is used for the detection of L-alanine-aminopeptidase in microorganisms. L-Alanine aminopeptidase is an enzyme localized in the bacterial cell wall which cleaves N-terminal L-alanine from various peptides. It is found almost exclusively in Gram-negative microorganisms. Studied Gram-positive or Gram-variable microorganisms show no or very weak activity. The aminopeptidase test is a reliable method for determining the Gram behavior and differentiating Gram-positive from Gram-negative bacteria. It does not replace Gram-staining, as it cannot show morphology.

Application

Aminopeptidase Test has been used to perform a Gram analysis of bacteria isolated from Scleroderma citrinum mycorrhizae, the mycorrhizosphere and bulk soil.

For the detection of L-alanine-aminopeptidase in microorganisms.
L-Alanine aminopeptidase is an enzyme localized in the bacterial cell wall which cleaves N-terminal L-alanine from various peptides. It is found almost exclusively in Gram-negative microorganisms. Studied Gram-positive or Gram-variable microorganisms show no or very weak activity. The aminopeptidase test is a reliable method for determining the Gram behavior. It does not replace Gram-staining, as it cannot show morphology.

Components

50 Test Strips (the reaction zone of each strip contains 0.5 μmol L-alanine-4-nitroanilide and buffering agents)

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves