MABN2709

Anti-LRAT Antibody, clone 49569

• Synonym(s): lecithin retinol acyl transferase, retinyl ester synthase
• UNSPSC Code: 12352203

Properties

• biological source: mouse
• Quality Level: 200
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 49569, monoclonal
• mol wt: calculated mol wt 25.820 kDa; observed mol wt ~24 kDa
• purified by: using protein G
• species reactivity: mouse
• packaging: antibody small pack of 100
• technique(s): immunofluorescence: suitable; immunohistochemistry: suitable; western blot: suitable
• isotype: IgG2aκ
• epitope sequence: Cytoplasmic domain
• Protein ID accession no.: NP_076113
• UniProt accession no.: Q9JI60
• storage temp.: 2-8°C
• Gene Information: mouse ... Lrat(79235)

Description

Specificity

Clone 49569 is a mouse monoclonal antibody that detects LRAT. It targets an epitope within 91 amino acids from the cytoplasmic domain of the protein.

Immunogen

His-tagged recombinant mouse LRAT fragment corresponding to 91 amino acids from the cytoplasmic domain of mouse LRAT.

Application

Quality Control Testing

Evaluated by Western Blotting in mouse retina lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected LRAT in mouse retina tissue lysate.

Tested Applications

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected LRAT in Retinal pigment epithelium (RPE) microsome lysate.

Immunofluorescence Analysis: A representative lot detected LRAT in Immunofluorescence applications (Batten, M.L., et al. (2004). J. Biol. Chem. 279(11):10422-32; Moise, A.R., et al. (2007). J Biol Chem. 282(3):2081-90).

Immunohistochemistry Analysis: A representative lot detected LRAT in Immunohistochemistry applications (Batten, M.L., et al. (2004). J. Biol. Chem. 279(11):10422-32; Moise, A.R., et al. (2007). J Biol Chem. 282(3):2081-90).

Western Blotting Analysis: A representative lot detected LRAT in Western Blotting applications (Batten, M.L., et al. (2004). J Biol Chem. 279(11):10422-32; Moise, A.R., et al. (2007). J. Biol. Chem. 282(3):2081-90).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Target description

Lecithin retinol acyltransferase (UniProt: Q9JI60) (also known as EC:2.3.1.135) is encoded by the Lrat gene (Gene ID:79235) in mouse. Lecithin-retinol acyltransferase (LRAT) is an essential enzyme involved in the metabolism of retinoids, particularly vitamin A. LRAT contains a conserved domain crucial for its enzymatic activity. The primary function of LRAT is to catalyze the esterification of retinol to produce retinyl esters, a process vital for the storage and transport of vitamin A within the body. LRAT′s plays a role in the visual cycle as it contributes to the regeneration of visual pigments in the retina. The expression of LRAT is regulated by dietary vitamin A levels, and it is found in various tissues, including the liver, lungs, testis, and the eye. LRAT is primarily localized to the endoplasmic reticulum. Mutations in the LRAT gene have been linked to a form of retinitis pigmentosa, an inherited retinal disease associated with progressive vision loss.

Physical form

Purified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Reconstitution

1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommended storage: +2°C to +8°C.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.