MABN1182
Anti-Glutamine Synthetase Antibody, clone 11A3.1
clone 11A3.1, from mouse
Synonym(s):
Glutamine synthetase, Glutamate-ammonia ligase, Glutamate decarboxylase, GS
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
mouse
Quality Level
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
11A3.1, monoclonal
species reactivity
human, rat
technique(s)
immunohistochemistry: suitable (paraffin)
western blot: suitable
isotype
IgG2aκ
NCBI accession no.
UniProt accession no.
shipped in
ambient
target post-translational modification
unmodified
Gene Information
human ... GLUL (2752)
rat ... Glul(24957)
General description
Glutamine synthetase (EC 4.1.1.15, EC 6.3.1.2; UniProt P09606; also known as Glutamate-ammonia ligase, Glutamate decarboxylase, GS) is encoded by the Glul (also known as Glns) gene (Gene ID 24957) in rat species. Glutamine synthetase (GS) plays an essential role in nitrogen metabolism by catalyzing ATP-dependent condensation of glutamate and ammonia to form glutamine. Three classes of GS exist, class I enzymes (GSI) are specific to prokaryotes and exist as oligomers of 12 identical subunits, class II enzymes (GSII) exist as decamer of identical subunits in eukaryotes and in some bacteria, while class III enzymes (GSIII) function as a double-ringed dodecamer of identical chains found in bacteroides fragilis and in butyrivibrio fibrisolvens. GS is present predominantly in the brain, kidneys, and liver in mammals. In the brain, GS is found primarily in astrocytes, where it participates in the the detoxification of ammonia via assimilation and termination of neurotransmitter signals via recyclization. c-Myc promotes glutamine usage by upregulating glutaminase (GLS), which converts glutamine to glutamate in the TCA cycle, while in some cancer cells Myc-mediated thymine DNA glycosylase (TDG) transcription leads to GS upregulation due to TDG-dependent demethylation of the GS promoter. GS upregulation promotes cancer cell survival under glutamine limitation, while GS silencing decreases cancer cell proliferation and xenograft tumor growth.
Specificity
Clone 11A3.1 targets an internal epitope within the C-terminal half of human and rat glutamine synthetase.
Immunogen
GST-tagged recombinant rat glutamine synthetase internal fragment.
Application
Anti-Glutamine Synthetase, clone 11A3.1, Cat. No. MABN1182, is a highly specific mouse monoclonal antibody that targets Glutamine Synthetase, validated for use in Immunohistochemistry (Paraffin) and Western Blotting.
Research Category
Neuroscience
Neuroscience
Western Blotting Analysis: A 1:20,000 dilution of from a representative lot detected Glutamine synthetase in 10 µg of human brain tissue lysate.
Immunohistochemistry Analysis: A 1:1000 dilution from a representative lot detected Glutamine synthetase in human cerebellum, cerebral cortex, and tonsil tissue sections.
Immunohistochemistry Analysis: A 1:1000 dilution from a representative lot detected Glutamine synthetase in human cerebellum, cerebral cortex, and tonsil tissue sections.
Quality
Evaluated by Western Blotting in rat brain tissue lysate.
Western Blotting Analysis: A 1:20,000 dilution of this antibody detected Glutamine synthetase in 10 µg of rat brain tissue lysate.
Western Blotting Analysis: A 1:20,000 dilution of this antibody detected Glutamine synthetase in 10 µg of rat brain tissue lysate.
Target description
~42 kDa observed. 42.06 kDa (human) and 42.27 kDa (rat) calculated. Uncharacterized bands may be observed in some lysate(s).
Physical form
Format: Purified
Protein G purified.
Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4) 150 mM NaCl with 0.05% sodium azide
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Other Notes
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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