MABF2072

Anti-MAdCAM-1 Antibody, clone MECA-367

clone MECA-367, from rat

• Synonym(s): Mucosal addressin cell adhesion molecule 1, mMAdCAM-1
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: rat
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: MECA-367, monoclonal
• species reactivity: mouse
• packaging: antibody small pack of 25 μg
• technique(s): immunohistochemistry: suitable (paraffin); neutralization: suitable; western blot: suitable
• isotype: IgG2aκ
• NCBI accession no.: XP_006513356.1
• UniProt accession no.: Q61826
• target post-translational modification: unmodified
• Gene Information: mouse ... Madcam1(17123)

Description

General description

Mucosal addressin cell adhesion molecule 1 (UniProt: Q61826; also known as MAdCAM-1, mMAdCAM-1) is encoded by the Madcam1 gene (Gene ID: 17123) in murine species. MAdCAM-1 is a cell adhesion leukocyte receptor that helps to direct lymphocyte traffic into mucosal tissues including the Peyer patches and the intestinal lamina propria. It is highly expressed on high endothelial venules (HEV) of organized intestinal lymphoid tissues and mesenteric lymph nodes, and in the lamina propria of the intestine. Its expression is also observed in brain endothelioma cells and mucosal tissues during chronic inflammation. Aberrant expression of MAdCAM-1 has also been reported in the inflamed pancreas. MAdCAM-1 can bind both the integrin alpha-4/beta-7 and L-selectin and is involved in regulating both the passage and retention of leukocytes. It is synthesized with a signal peptide (aa 1-21), which is subsequently cleaved off in mature form. MAdCAM-1 has an extracellular domain (aa 22-109), a transmembrane domain (aa 365-385), and a cytoplasmic domain (aa 386-405). It also contains three Ig-like domains (aa 22-109; 110-227; 258-357) and a Mucin-like region (aa 221-257). Two isoforms of MAdCAM-1 have been described that are produced by alternative splicing. Both isoforms can adhere to integrin alpha-4/beta-7. Isoform 2 that lacks the mucin-like domain is believed to be specialized in supporting integrin alpha-4/beta-7-dependent adhesion strengthening, independent of L-selectin binding. Interaction between integrin alpha 4 and MAdCAM-1 is considered to be important for the homing of diabetogenic T cells to the pancreas. (Ref.: Savinov, AY., et al. (2003). J. Exp. Med. 197(5); 643-656; Rivera-Nieves, J., et al. (2005). J. Immunol. 174(4); 2343-2352).

Specificity

Clone MECA-367 detects Mucosal addressin cell adhesion molecule 1 (MAdCAM-1) in murine cells.

Immunogen

Endothelial cells isolated from BALB/c mouse mesenteric and peripheral lymph nodes.

Application

Anti-MAdCAM-1, clone MECA-367, Cat. No. MABF2072, is a rat monoclonal antibody that detects murine Mucosal addressin cell adhesion molecule 1 (MAdCAM-1) and has been tested for use in Immunohistochemistry (Paraffin), Inhibition/Function assays, Neutralizing, and Western Blotting.

Research Category
Inflammation & Immunology

Western Blotting Analysis: A representative lot detected MAdCAM-1 in Western Blotting applications (Streeter, P.R., et. al. (1988). Nature. 331(6151):41-6).

Immunohistochemistry (Paraffin) Analysis: A representative lot detected MAdCAM-1 in Immunohistochemistry applications (Streeter, P.R., et. al. (1988). Nature. 331(6151):41-6).

Immunohistochemistry Analysis: A representative lot detected MAdCAM-1 in Immunohistochemistry applications (Streeter, P.R., et. al. (1988). Nature. 331(6151):41-6; Rantakari, P., et. al. (2016). Nature. 538(7625):392-396).

Neutralizing Analysis: A representative lot neutralizes the action on MAdCAM-1 and reduces chronic ileitis (Rivera-Nieves, J., et. al. (2005). J Immunol. 174(4):2343-52).


Inhibits Activity/Function: A representative lot almost completely inhibited lymphocyte migration to Peyer s patches and also blocked the adhesion of the mucosal high endothelial venules (HEV) binding to T cell lymphoma TK1. (Streeter, P.R., et. al. (1988). Nature. 331(6151):41-6; Briskin, M.J., et. al. (1993). Nature. 363(6428):461-4).

Quality

Evaluated by Immunohistochemistry (Paraffin) in mouse colon tissue sections.

Immunohistochemistry (Paraffin) Analysis: A 1:50 dilution of this antibody detected MAdCAM-1 in mouse colon tissue sections.

Target description

43.65 kDa calculated.

Physical form

Format: Purified

Protein G purified

Purified rat monoclonal antibody IgG2a in PBS without azide.

Storage and Stability

Stable for 1 year at -20°C from date of receipt.

Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.