MABE339
Anti-p53 (wild type) Antibody, clone PAb1620
clone Pab1620, from mouse
Synonym(s):
Cellular tumor antigen p53, Tumor suppressor p53
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
biological source
mouse
Quality Level
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
Pab1620, monoclonal
species reactivity
human, mouse
technique(s)
immunohistochemistry: suitable
western blot: suitable
isotype
IgG2aκ
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... TP53(7157)
General description
Wild-type p53 is present in the nucleus of all normal mammalian cells where it appears to be involved in the regulation of cell proliferation. The normal protein has a very short half-life and is present in only minute amounts in normal tissues and cells. In contrast, mutant p53 protein produced by malignant cells is usually a product of a point mutation in the p53 gene leading to substitution of a single amino acid that significantly prolongs the half-life of the protein. The accumulation of high levels of p53 is a potential novel marker for malignancy.
Immunogen
Mouse VLM tumor cells.
Application
Anti-p53 (wild type) Antibody, clone PAb1620 is a Mouse Monoclonal Antibody for detection of p53 (wild type) also known as Cellular tumor antigen p53, Tumor suppressor p53 & has been validated in WB & IHC.
Immunohistochemistry Analysis: A 1:50 dilution of this antibody detected p53 in normal human brain tissue.
Immunoprecipitation Analysis: A representative lot from an independent laboratory detected p53 in IP (Lu, X., et al. (1992). Cell. 70(1):153-161.).
Immunohistochemistry Analysis: A representative lot from an independent laboratory detected p53 in dorsal skin samples from animal trunks (Kramata, P., et al. (2005). Cancer Res. 65(9):3577-3585.) and in certain human cancer tissues (Cordon-Cardo, C., et al, (1991). 51(23 Pt 1):6372-6380.).
Immunoprecipitation Analysis: A representative lot from an independent laboratory detected p53 in IP (Lu, X., et al. (1992). Cell. 70(1):153-161.).
Immunohistochemistry Analysis: A representative lot from an independent laboratory detected p53 in dorsal skin samples from animal trunks (Kramata, P., et al. (2005). Cancer Res. 65(9):3577-3585.) and in certain human cancer tissues (Cordon-Cardo, C., et al, (1991). 51(23 Pt 1):6372-6380.).
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Cell Cycle, DNA Replication & Repair
Cell Cycle, DNA Replication & Repair
Quality
Evaluated by Western Blot in mouse brain tissue lysate.
Western Blot Analysis: 1 µg/mL of this antibody detected p53 in mouse brain tissue lysate.
Western Blot Analysis: 1 µg/mL of this antibody detected p53 in mouse brain tissue lysate.
Target description
~53 kDa observed
Physical form
Format: Purified
Protein G Purified
Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
Mouse brain tissue lysate
Mouse brain tissue lysate
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Ras-induced hyperplasia occurs with mutation of p53, but activated ras and myc together can induce carcinoma without p53 mutation.
Lu, X, et al.
Cell, 70, 153-161 (1992)
Patches of mutant p53-immunoreactive epidermal cells induced by chronic UVB Irradiation harbor the same p53 mutations as squamous cell carcinomas in the skin of hairless SKH-1 mice.
Kramata, Pavel, et al.
Cancer Research, 65, 3577-3585 (2005)
J Muret et al.
Annals of oncology : official journal of the European Society for Medical Oncology, 19(4), 793-800 (2007-12-11)
Recombinant tumor necrosis factor-alpha (TNF-alpha) combined to melphalan is clinically administered through isolated limb perfusion (ILP) for regionally advanced soft tissue sarcomas of the limbs. In preclinical studies, wild-type p53 gene is involved in the regulation of cytotoxic action of
M W Ravera et al.
Oncogene, 16(15), 1993-1999 (1998-05-20)
Monoclonal antibody PAb1620 recognizes a conformational epitope on the transcription factor p53 and, upon binding, allosterically inhibits p53 binding to DNA. A highly diverse (1.5 x 10(10) members) phage-displayed library of peptides containing 40 random amino acids was used to
Expression of HLA-A,B,C antigens on primary and metastatic tumor cell populations of human carcinomas.
Cordon-Cardo, C, et al.
Cancer Research, 51, 6372-6380 (1991)
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