MAB1407P
Anti-Macrophages/Granulocytes Antibody, clone OX-41
clone OX-41, Chemicon®, from mouse
Synonym(s):
CD172 antigen-like family member A, CD172a antigen, Inhibitory receptor SHPS-1, Macrophage fusion receptor, MyD-1 antigen, SHP substrate 1, SHP substrate-1, Signal-regulatory protein alpha-1, signal-regulatory protein alpha
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
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biological source
mouse
Quality Level
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
OX-41, monoclonal
purified by
affinity chromatography
species reactivity
mouse, human, rat
manufacturer/tradename
Chemicon®
technique(s)
flow cytometry: suitable
immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable
isotype
IgG2a
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... SIRPA(140885)
Related Categories
General description
Macrophages and granulocytes express Signal regulatory protein (SIRP), which is a transmembrane glycoprotein involved in the down regulation of tyrosine kinase receptor signal transduction induced by cell adhesion, growth factors or insulin. They are immunoglobulin-like cell surface receptor for CD47. By CD47 binding there is a negative regulation of phagocytosis, mast cell activation and dendritic cell activation that prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells.
Specificity
Expected to cross-react with rat and mouse.
MAB1407P recognizes rat SIRP (Signal regulatory protein), which is expressed selectively by myeloid cells and by neurons. This monoclonal binds to a different epitope on SIRP than ED9. It does not block the interaction of CD172a and CD47.
Labels most macrophages including resident peritoneal, alveolar and activated macrophages. Recognition of other macrophages is limited to only occasional kupffer cells. In addition it labels granulocytes and a population of dendritic cells. It also labels brain extensively.
Labels most macrophages including resident peritoneal, alveolar and activated macrophages. Recognition of other macrophages is limited to only occasional kupffer cells. In addition it labels granulocytes and a population of dendritic cells. It also labels brain extensively.
Immunogen
Rat peritoneal macrophages.
Application
Immunohistochemistry:
Frozen sections and Formalin fixed, paraffin embedded tissue sections (requires trypsin or pronase pre-treatment).
Western Blotting:
A previous lot of this antibody was used in western blot.
Flow cytometry:
1:100-1:200 of a previous lot was used. (Use 10 μL of working dilution for 10^6 cells in 100 μL).
Optimal working dilutions must be determined by the end user.
Frozen sections and Formalin fixed, paraffin embedded tissue sections (requires trypsin or pronase pre-treatment).
Western Blotting:
A previous lot of this antibody was used in western blot.
Flow cytometry:
1:100-1:200 of a previous lot was used. (Use 10 μL of working dilution for 10^6 cells in 100 μL).
Optimal working dilutions must be determined by the end user.
Research Category
Inflammation & Immunology
Inflammation & Immunology
Research Sub Category
Inflammation & Autoimmune Mechanisms
Inflammation & Autoimmune Mechanisms
This Anti-Macrophages/Granulocytes Antibody, clone OX-41 is validated for use in FC, IC, IH, IH(P), WB for the detection of Macrophages/Granulocytes.
Quality
Routinely evaluated by Immunocytochemistry on Macrophages/Granulocytes.
Immunocytochemistry Analysis:
Confolcal fluorescent analysis of Macrophages/ Granulocytes using anti-Macrophages/ Granulocytes mouse monoclonal antibody.
Immunocytochemistry Analysis:
Confolcal fluorescent analysis of Macrophages/ Granulocytes using anti-Macrophages/ Granulocytes mouse monoclonal antibody.
Target description
56 kDa
Linkage
Replaces: MAB1407
Physical form
ImmunoAffinity Purified
Purified mouse monoclonal IgG2a in buffer containing 0.09% sodium azide.
Storage and Stability
Stable for 6 months at 2-8°C in undiluted aliquots from date of receipt.
Analysis Note
Control
Macrophages, monocytes, lymphoid organs.
Macrophages, monocytes, lymphoid organs.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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