MAB1285

Anti-HLA-B27 Antibody, clone HLA.ABC.m3

clone HLA.ABC.m3, Chemicon^®, from mouse

• Synonym(s): Anti-AS, Anti-B-4901, Anti-HLAB
• UNSPSC Code: 12352203
• eCl@ss: 32160702

Properties

• biological source: mouse
• Quality Level: 100
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: HLA.ABC.m3, monoclonal
• species reactivity: human
• manufacturer/tradename: Chemicon^®
• technique(s): flow cytometry: suitable; immunocytochemistry: suitable
• isotype: IgG2a
• NCBI accession no.: NM_005514.5
• UniProt accession no.: P01889
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... HLA-B(3106)

Description

General description

The previously assigned protein identifier P03989 has been merged into P01889. Full details can be found on the UniProt database.

Specificity

This antibody binds strongly to the HLA-B27 antigen. It also binds less strongly to the HLA-B7 antigen. Affinity studies by Scatchard analysis showed that the antibody has a higher affinity for HLA-B27 (9.7x108M-1) than for HLA-B7

(9.5x107M-1).

Cell reactivity:

Flow cytometric analysis of lymphocyte staining for HLA-B27 gave the following results:



Subjects Fluorescence Intensity

Heterozygous HLA-B27+ Strong

Homozygous HLA-B7+ Strong

Heterozygous HLA-B7+ Faint

Non B27, Non B7 Negative

Application

This Anti-HLA-B27 Antibody, clone HLA.ABC.m3 is validated for use in FC, IC for the detection of HLA-B27.

This antibody is useful in the investigation of HLA-B27 in diseases such as ankylosing spondylitis and anterior uveitis.

SUGGESTED USAGE DILUTION

1. Flow cytometry and indirect immunofluorescence 1:60 to 1:120

Dilute with isotonic buffer. Use 50 μl of diluted antibody per 1 x 10E6 peripheral blood mononuclear cells (PBMC) in 100 μl buffer.

2. Cytotoxicity testing 1:25.

Physical form

Format: Purified

The antibody is supplied in phosphate-buffered saline, pH 7.4, containing 0.2% bovine serum albumin and 0.1% sodium azide. The characteristics of each lot are tested by flow cytometry.

Storage and Stability

Store at 2 to 8°C. For prolonged periods, store below -20°C in undiluted aliquots. AVOID REPEATED FREEZE/THAW CYCLES.

WARNING: The monoclonal reagent solution contains 0.1% sodium azide as a preservative. Due to potential hazards arising from the build up of this material in pipes, spent reagent should be disposed of with liberal volumes of water.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable