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Key Documents

AB5714

Sigma-Aldrich

Anti-Hey 1/HRT1 Antibody

Chemicon®, from rabbit

Synonym(s):

Anti-BHLHb31, Anti-HERP2, Anti-HESR1, Anti-HRT-1, Anti-NERP2, Anti-OAF1, Anti-hHRT1

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

saturated ammonium sulfate (SAS) precipitated

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

human, mouse

manufacturer/tradename

Chemicon®

technique(s)

immunohistochemistry: suitable
western blot: suitable

NCBI accession no.

UniProt accession no.

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

human ... HEY1(23462)

Related Categories

Specificity

Recognizes Hey 1 / HRT1, a Helix-loop-helix class of transcription factor.

Immunogen

Synthetic peptide, amino acids 207-220 of human Hey 1 / HRT1.

Application

Anti-Hey 1/HRT1 Antibody detects level of Hey 1/HRT1 & has been published & validated for use in WB, IH.
Research Category
Neuroscience
Research Sub Category
Developmental Neuroscience

Neuronal & Glial Markers
Western blot: 1:200-1:1,000 using ECL.

Immunohistochemistry: 1:200-1:1,000.

Optimal working dilutions must be determined by the end user.

Physical form

Precipitated antibody in a solution of 50% saturated ammonium sulfate and PBS containing no preservatives.

Storage and Stability

Maintain unopened vial at -70°C for up to 6 months. Avoid repeated freeze/thaw cycles.

The rehydrated antibody solutions can be stored undiluted at 2-8°C for 2 months without any significant loss of activity. Note, the solution is not sterile, thus care should be taken if product is stored at 2-8°C.


For storage at -20°C, the addition of an equal volume of glycerol can be used, however, it is recommended that ACS grade or higher glycerol be used, as significant loss of activity can occur if the glycerol used is not of high quality.

For freezing, it is recommended that the rehydrated antibody solution be further diluted 1:1 with a 2% BSA (fraction V, highest-grade available) solution made with the rehydration buffer. The resulting 1% BSA/antibody solution can be aliquoted and stored frozen at -70°C for up to 6 months. Avoid repeated freeze/thaw cycles.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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