345386

Glucose Oxidase, Aspergillus niger, Recombinant

Catalyzes the oxidation of β-D-glucose to D-glucono-ω-lactone and hydrogen peroxide. Also catalyzes the conversion of D-glucono-ω-lactone to gluconic acid.

• Synonym(s): Glucose Oxidase, Aspergillus niger, Recombinant
• CAS Number: 9001-37-0
• Enzyme Commission number: 1.1.3.4 (BRENDA, IUBMB)
• MDL number: MFCD00131182
• UNSPSC Code: 12352202

Properties

• Quality Level: 100
• form: lyophilized solid
• specific activity: ≥250 units/mg solid
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze
• color: yellow-brown
• solubility: 0.1 M phosphate buffer, pH 7.0: 5 mg/mL
• foreign activity: Amylase ≤0.1%; catalase ≤20 U/mg (dry weight); sucrase ≤0.1%
• shipped in: ambient
• storage temp.: 2-8°C
• InChI: 1S/C6H12O6/c7-1-2-3(8)4(9)5(10)6(11)12-2/h2-11H,1H2/t2-,3-,4+,5-,6-/m1/s1
• InChI key: WQZGKKKJIJFFOK-VFUOTHLCSA-N

Description

General description

Note: 1 KU = 1000 units.

Recombinant, Aspergillus niger glucose oxidase. Catalyzes the oxidation of β-D-glucose to D-glucono-ω-lactone and hydrogen peroxide. Also catalyzes the conversion of D-glucono-ω-lactone to gluconic acid. Each molecule of enzyme contains 2 molecules of FAD, which are essential for enzyme activity. Optimal pH = 6.5 with >95% maximal activity at pH 6.0-7.0. Useful for enzymatic assay of glucose. May also be conjugated to antibodies or antigens for use in ELISA procedures.

Recombinant, Aspergillus niger glucose oxidase. Catalyzes the oxidation of β-D-glucose to D-glucono-ω-lactone and hydrogen peroxide. Also catalyzes the conversion of D-glucono-ω-lactone to gluconic acid. Useful for enzymatic assay of glucose concentration.

Warning

Toxicity: Standard Handling (A)

Unit Definition

One unit is defined as the amount of enzyme that will oxidize 1.0 mmol of β-D-glucose per minute at 25°C, pH 7.0.

Reconstitution

Following reconstitution, aliquot and refrigerate (4°C). Stock solutions are stable for up to 3 months at 4°C.

Other Notes

Heinz, F. and Beushausen, T.W. 1981. J. Clin. Chem. Clin. Biochem. 19, 977.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Pictograms: GHS08
• Signal Word: Danger
• Hazard Statements: H334
• Precautionary Statements: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable