124008

Akt Inhibitor II

The Akt Inhibitor II controls the biological activity of Akt. This small molecule/inhibitor is primarily used for Phosphorylation & Dephosphorylation applications.

• Synonym(s): Akt Inhibitor II, SH-5
• Empirical Formula (Hill Notation): C₂₉H₅₉O₁₀P
• Molecular Weight: 598.75
• UNSPSC Code: 12352200
• NACRES: NA.77

Properties

• Quality Level: 100
• Assay: ≥98% (NMR)
• form: solid
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze
• color: white
• solubility: DMSO: 10 mg/mL
• shipped in: ambient
• storage temp.: −20°C

Description

General description

A cell permeable, reversible, and substrate competitive phosphatidylinositol analog that inhibits the activation of Akt and select downstream substrates without decreasing phosphorylation of PDK-1 or other kinases downstream of Ras, such as MAPK. Also acts as a potent inducer of apoptosis and selectively kills a variety of cancer cell lines that contain high levels of active Akt. When used at a concentration of 10 µM, it is reported to activate p38α/SAPK2α MAP kinase. At 10 µM it will also inhibit ~50% of PKA activity.

A cell permeable, reversible, and substrate competitive phosphatidylinositol analog that inhibits the activation of Akt and selected downstream substrates without affecting the phosphorylation of PDK-1 and other downstream kinases. Decreases phosphorylation of Akt without affecting the total Akt level. Reported to be a potent inducer of apoptosis and to selectively kill a variety of cancer cell lines expressing high levels of active Akt. When used at a concentration of 10 µM, it is reported to activate p38α/SAPK2α MAP kinase. At 10 µM it will also inhibit ~50% of PKA activity.

Biochem/physiol Actions

Cell permeable: yes

Primary Target
Akt

Product does not compete with ATP.

Reversible: yes

Packaging

Packaged under inert gas

Warning

Toxicity: Standard Handling (A)

Reconstitution

Following reconstitution aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Other Notes

Gills, J.J., et al. 2007. J. Biol. Chem.in press.
Kozikowski, A.P., et al. 2003. J. Am. Chem. Soc.125, 1144.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable