1.03867
GranuCult® plus Deoxycholate Hydrogen sulfide Lactose Agar
acc. SAKAZAKI
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About This Item
UNSPSC Code:
41106213
NACRES:
NA.74
Recommended Products
Quality Level
form
powder
technique(s)
microbiological culture: suitable
application(s)
food and beverages
pharmaceutical
suitability
nonselective for enterobacteriaceae
General description
GranuCult® plus Deoxycholate Hydrogen sulfide Lactose (DHL) Agar is a nutrient rich medium to detect and isolate Gram-negative bacteria, from different materials. H2S production is indicated by a blackening of the colonies due to formation of iron sulfide. Although Proteus is H2S-positive, its colonies are not black.
Colonies of Proteus, Morganella, Rettgerella and Providencia are, however, surrounded by dark brown zones, which occur, because these species act on the phenylalanine of the peptone to produce phenylpyruvate, which forms an iron complex with iron(III) ions.
The sucrose content of the medium permits differentiation of weakly lactose-positive or lactose-negative, sucrose-positive species from sucrose- and lactose-negative Enterobacteriaceae.
The deoxycholate largely suppresses the growth of Gram-positive bacteria and prevents the swarming of Proteus species. This medium provides a rich nutrient base and contains a relatively low concentration of the inhibitor deoxycholate. These properties permit growth of even fastidious strains of Salmonella and Shigella. The colonies formed are considerably larger than those found on other selective culture media. Proteus, Morganella, Rettgerella and Providencia can be differentiated from Salmonella.
Neutral Red is a pH indicator and agar is the solidifying agent. .
Colonies of Proteus, Morganella, Rettgerella and Providencia are, however, surrounded by dark brown zones, which occur, because these species act on the phenylalanine of the peptone to produce phenylpyruvate, which forms an iron complex with iron(III) ions.
The sucrose content of the medium permits differentiation of weakly lactose-positive or lactose-negative, sucrose-positive species from sucrose- and lactose-negative Enterobacteriaceae.
The deoxycholate largely suppresses the growth of Gram-positive bacteria and prevents the swarming of Proteus species. This medium provides a rich nutrient base and contains a relatively low concentration of the inhibitor deoxycholate. These properties permit growth of even fastidious strains of Salmonella and Shigella. The colonies formed are considerably larger than those found on other selective culture media. Proteus, Morganella, Rettgerella and Providencia can be differentiated from Salmonella.
Neutral Red is a pH indicator and agar is the solidifying agent. .
Application
GranuCult® plus Deoxycholate Hydrogen sulfide Lactose (DHL) Agar is used to isolate medium to detect and isolate Gram-negative bacteria from different materials. The gram-positive bacterial growth is inhibited by the medium properties that accompany the test sample.
Other Notes
DHL (Deoxycholate Hydrogen sulfide Lactose) agar acc. to SAKAZAKI is also known as Sakazaki DHL agar.
Footnote
We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.
Legal Information
GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
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