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Key Documents

07-590

Sigma-Aldrich

Anti-Ago 2 Antibody

Upstate®, from rabbit

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

polyclonal

species reactivity

human

packaging

antibody small pack of 25 μg

manufacturer/tradename

Upstate®

technique(s)

western blot: suitable

isotype

IgG

NCBI accession no.

UniProt accession no.

shipped in

ambient

target post-translational modification

unmodified

Gene Information

human ... AGO2(27161)

General description

This gene encodes a member of the Argonaute family of proteins which play a role in RNA interference. The encoded protein is highly basic, and contains a PAZ domain and a PIWI domain. It may interact with dicer1 and play a role in short-interfering-RNA-mediated gene silencing.

Provides endonuclease activity to RNA-induced silencing complexes (RISC). Cleaves siRNA/mRNA heteroduplexes bound to RISC. Essential for embryonic development as well as RNA-mediated gene silencing (RNAi).

Specificity

Recognizes Ago 2, also known as eukaryotic translation initiation factor 2C, 2 (EIF2C 2), MGC3183 and Q10.

Immunogen

GST fusion protein corresponding to residues 7-48 of human argonaute 2 (Ago2).

Application

Anti-Ago 2 Antibody is a Rabbit Polyclonal Antibody for detection of Ago 2 also known as argonaute 2 & has been tested in WB.

Quality

Routinely evaluated by immunoblot in RIPA and cytosolic lysates from HeLa cells.

Target description

100 kDa

Physical form

Format: Purified

Analysis Note

Control
HeLa cell lysate, HL-60 cell lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class Code

10 - Combustible liquids

WGK

WGK 1


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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