G005619

LC/MS/MS Analysis of Carbendazim in Orange Juice (Spiked) on Ascentis^® Express C18 after Supel^™ QuE PSA/C18 QuEChERS Cleanup

application for HPLC, application for SPE

Properties

• technique(s): HPLC: suitable; solid phase extraction (SPE): suitable
• test parameters: sample preparation: Dispersive (QuEChERS); sample/matrix: orange juice spiked with carbendazim at 1 ppb; extraction tube: Supel^™ QuE Citrate (EN) Tube (55227-U); extraction process: add 10 mL of orange juice with pulp to a 50 mL empty extraction tube (55248-U); add carbendazim at 1 ppb; add 10 mL acetonitrile; (shake for 1 minute; add contents of citrate extraction tube (55227-U); shake for 1 min; centrifuge at 3200 rpm for 5 minutes); clean-up tube: Supel-Q^™ PSA/C18 (55288-U); clean-up process: transfer 0.7 mL of the acetonitrile layer to the PSA/C18 cleanup tube (55288-U); shake 1 minute; (centrifuge for 3 minutes at 3400 rpm; remove 0.25 mL of the supernatant; mix with 0.25 mL water for LC-MS analysis); column: Ascentis Express C18, 5 cm x 2.1 mm I.D., 2.7 μm particles (53822-U); mobile phase: [A] 10 mM ammonium acetate in water; [B] 10 mM ammonium acetate in methanol; gradient: 0-1 min: 30% B; 1.5-3.5 min: 100% B; 3.5-7 min: 30% B; flow rate: 0-1 min: 0.3 mL/min; 1.5-7 min: 0.5 mL/min; column temp.: 30 °C; detector: MS, ESI(+), MRM, m/z 192/160, 192/132; injection: 5 μL
• suitability: application for HPLC; application for SPE
• application(s): food and beverages

Description

Analysis Note

Simple and fast extraction and analytical method were developed for analysis of fungicide carbendazim in orange juice samples. QuEChERS extraction method was used with PSA/C18 cleanup. Ascentis Express C18 column chromatography was coupled to MS/MS detection with limits of detection at 0,1 ppb.

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

Supel is a trademark of Sigma-Aldrich Co. LLC

Analyte

Carbendazim, PESTANAL^®, analytical standard