821982

TSKgel^® Amide-80 HPLC Column

phase carbamoyl, L × I.D. 25 cm × 4.6 mm, 5 μm particle size

• Synonym(s): TSKgel^® Amide-80 HR
• UNSPSC Code: 41115711
• eCl@ss: 32110501

Properties

• material: stainless steel hardware
• Agency: suitable for USP L68
• description: Amide-80 HR
• product line: TSKgel^®
• manufacturer/tradename: Tosoh 21982
• parameter: 0-100% organic solvent; 10-80 °C temperature; 150 bar max. pressure
• technique(s): HPLC: suitable
• L × I.D.: 25 cm × 4.6 mm
• surface area: 450 m²/g
• matrix: silica particle platform
• matrix active group: carbamoyl phase
• particle size: 5 μm
• pore size: 100 Å
• operating pH: 2.0-7.5
• separation technique: hydrophilic interaction (HILIC)

Description

General description

TSKgel Amide-80 columns are now also available packed with 2 μm spherical silica particles, offering higher resolution and faster analysis with equivalent retention and selectivity as TSKgel Amide-80, 3 μm columns. This allows for smooth method transfer from a 3 μm to a 2 μm column. In addition, TSKgel Amide-80, 2 μm columns more highly retain hydrophilic compounds than competitive amide columns on the market.
Packed with 2, 3, 5, or 10 μm spherical silica particles that are covalently bonded with carbamoyl groups, TSKgel Amide-80 columns are used for the analysis of saccharides, glycans, oligosaccharides, peptides and polar compounds from natural products. The amide stationary phase provides a unique selectivity under regular normal phase conditions or in the hydrophilic interaction mode of chromatography.
TSKgel Amide-80 HR, 5 μm columns offer higher specifications for plate count compared to traditional TSKgel Amide-80, 5 μm, 4.6 mm ID x 25 cm columns (N = > 18,000 vs. 8,000). This equates to more efficient chromatography.

Physical form

Shipped in 75% acetonitrile - 25% water.

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Legal Information

TSKgel is a registered trademark of Tosoh Corporation