ZRT1338
Anti-ST2/IL-33R Antibody, clone RMST-2 ZooMAb® Rat Monoclonal
recombinant, expressed in HEK 293 cells
Synonym(s):
EC:3.2.2.6, Interleukin-1 receptor-like 1, Interleukin-33 receptor alpha chain 1, Lymphocyte antigen 84, Protein ST2, Protein T1
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.43
Recommended Products
biological source
rat
Quality Level
recombinant
expressed in HEK 293 cells
conjugate
unconjugated
antibody form
purified antibody
antibody product type
primary antibodies
clone
recombinant monoclonal
description
clone RMST-2
recombinant, expressed in HEK 293 cells
product line
ZooMAb® learn more
form
lyophilized
mol wt
calculated mol wt 64.8 kDa
observed mol wt ~70 kDa
purified by
using protein G
species reactivity
mouse, human
packaging
antibody small pack of 25
greener alternative product characteristics
Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.
enhanced validation
recombinant expression
Learn more about Antibody Enhanced Validation
sustainability
Greener Alternative Product
technique(s)
flow cytometry: suitable
immunocytochemistry: suitable
western blot: suitable
isotype
IgG2aκ
Protein ID accession no.
UniProt accession no.
shipped in
ambient
storage temp.
2-8°C
Gene Information
mouse ... Il1rl1(17082)
General description
We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry. This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.
Specificity
Clone RMST-2 is a ZooMAb® rat recombinant monoclonal antibody that specifically detects ST2/IL-33R.
Immunogen
Mouse ST-2/IL1RL1.
Application
Quality Control Testing
Evaluated by Western Blotting in HEK293 cell lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected ST2/IL-33R in HEK293 cell lysate.
Tested Applications
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected ST2/IL-33R in L5178Y cells transfected with ST2/IL-33R expression vector.
Flow Cytometry Analysis: 0.1 µg from a representative lot detected ST2/IL-33R in one million L5178Y cells transfected with ST2/IL-33R expression vector.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Evaluated by Western Blotting in HEK293 cell lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected ST2/IL-33R in HEK293 cell lysate.
Tested Applications
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected ST2/IL-33R in L5178Y cells transfected with ST2/IL-33R expression vector.
Flow Cytometry Analysis: 0.1 µg from a representative lot detected ST2/IL-33R in one million L5178Y cells transfected with ST2/IL-33R expression vector.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Target description
Interleukin-1 receptor-like 1 (UniProt: P14719; also known as EC:3.2.2.6, Interleukin-33 receptor alpha chain 1, Lymphocyte antigen 84, Protein ST2, Protein T1) is encoded by the Il1rl1 (also known as Ly84, St2, Ste2) gene (Gene ID: 17082) in murine species. IL-33, a cytokine that is constitutively produced from by several structural and lining cells, transmits its signal through its receptor IL-33R (ST2), which is expressed on the surface of T helper (Th2) cells and group 2 lymphoid cells. IL-33R is a single-pass type I membrane glycoprotein, which is synthesized with a signal peptide (aa 1-26) that is subsequently cleaved off to produce the mature protein that contains an extracellular domain (aa 27-332), a transmembrane domain (aa 333-355), and a cytoplasmic domain (aa 356-567). The full-length IL-33R contains three Ig-like C2 type motifs that are responsible for extracellular ligand-binding. It also contains a TIR domain (aa 380-540) that is critical for initiating intracellular signaling. The IL-33/IL-33R axis plays a vital role in both innate and adaptive immunity and is involved in the development of multiple types of immune-related diseases. Two isoforms of IL-33R are described that are produced by alternative splicing. The soluble form (sST2) serves as a decoy receptor and lacks the transmembrane and cytoplasmic domains. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonal. (Ref.: Yi, X-M., et al. (2022). Cell Insight. 1; 100042).
Physical form
Purified recombinant rat monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.
Reconstitution
300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
Storage and Stability
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
Legal Information
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
11 - Combustible Solids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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