ZRB2704
Anti-SPNS2 Antibody, clone 1F4 ZooMAb® Rabbit Monoclonal
recombinant, expressed in HEK 293 cells
Synonym(s):
Protein spinster homolog 2, Sphingosine-1-phosphate transporter SPNS2
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About This Item
UNSPSC Code:
12352203
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biological source
rabbit
Quality Level
recombinant
expressed in HEK 293 cells
conjugate
unconjugated
antibody form
purified antibody
antibody product type
primary antibodies
clone
recombinant monoclonal
description
clone 1F4
recombinant, expressed in HEK 293 cells
product line
ZooMAb® learn more
form
lyophilized
mol wt
calculated mol wt 60 kDa
purified by
using Protein A
species reactivity
human
packaging
antibody small pack of 25
greener alternative product characteristics
Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.
enhanced validation
recombinant expression
Learn more about Antibody Enhanced Validation
technique(s)
affinity binding assay: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
isotype
IgG
epitope sequence
C-terminal half
Protein ID accession no.
UniProt accession no.
greener alternative category
, Aligned
shipped in
ambient
storage temp.
2-8°C
Gene Information
human ... SPNS2(124976)
Related Categories
General description
We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry. This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.
Specificity
Clone 1F4 is a ZooMAb® rabbit recombinant monoclonal antibody that specifically detect Sphingosine-1-phosphate transporter SPNS2. It targets an epitope within 23 amino acids from the internal region.
Immunogen
KLH-conjugated linear peptide corresponding to 23 amino acids from the internal region of human Sphingosine-1-phosphate transporter SPNS2.
Application
Quality Control Testing
Evaluated by Immunohistochemistry (Paraffin) in Human heart tissue sections.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution of this antibody detected SPNS2 in Human heart tissue sections.
Tested Applications
Affinity Binding Assay: A representative lot of this antibody bound SPNS2 peptide with a KD of 9.3 x 10-7 in an affinity binding assay.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected SPNS2 in ACHN cells.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Evaluated by Immunohistochemistry (Paraffin) in Human heart tissue sections.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution of this antibody detected SPNS2 in Human heart tissue sections.
Tested Applications
Affinity Binding Assay: A representative lot of this antibody bound SPNS2 peptide with a KD of 9.3 x 10-7 in an affinity binding assay.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected SPNS2 in ACHN cells.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Target description
Sphingosine-1-phosphate transporter SPNS2 (UniProt: Q8IVW8; also known as Protein spinster homolog 2, SPNS2) is encoded by the SPNS2 gene (Gene ID: 124976) in human. SPNS2, a member of the large family of non-ATP-dependent organic ion transporter, is a multi-pass membrane protein that serves as a lipid transporter and specifically mediates the export of sphingosine-1-phosphate (S1P) and sphinganine-1-phosphate. It regulates levels of S1P and the S1P gradient that exists between the high circulating concentrations of S1P and low tissue levels that control lymphocyte trafficking. It is required for the egress of T-cells from lymph nodes during an immune response by mediating S1P secretion, which generates a gradient that enables activated T-cells to access lymph. It is also essential for the egress of immature B-cells from the bone marrow. SPNS2 also plays a role in innate immunity and SPNS2 knockout animals show reduced IFN- responses to infections due to the inability of natural killer cells to egress from lymph nodes. The deletion of SPNS2 is reported to impair humoral immune responses to immunization, reduced sensitivity to antigen-induced airway inflammation and hypersensitivity and attenuated delayed-type contact hypersensitivity. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Chen, H., et al. (2023). Cell 186(12); 2644-2655; Spigiel, S., et al. (2019). J. Lipid Res. 60 (3); 484-489; Fang, V., et al. (2017). Nat. Immunol., 18; 15-25).
Physical form
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.
Reconstitution
300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
Storage and Stability
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
Legal Information
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
11 - Combustible Solids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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